Oligomeric adiponectin forms and their complexes in the blood of healthy donors and patients with type 2 diabetes mellitus

Autor:	Oleg A Nagibin, Alexey G. Katrukha, Ekaterina V Mironova, Olga V. Kolosova, Anastasiya V. Bereznikova, Andrei N. Kara, Ivan A Katrukha, Natalya S Zhuravleva, Vladimir L. Filatov, Kogan Ae
Rok vydání:	2013
Předmět:	Adult Male medicine.medical_specialty Immunoconjugates medicine.drug_class Clinical Biochemistry Immunology Plasma protein binding Monoclonal antibody Mice Internal medicine Diabetes mellitus medicine Immunology and Allergy Animals Humans Protein Isoforms Serum Albumin Aged biology Adiponectin business.industry Complement C1q Albumin Type 2 Diabetes Mellitus Antibodies Monoclonal Middle Aged medicine.disease Molecular biology Blot Molecular Weight Medical Laboratory Technology Endocrinology Diabetes Mellitus Type 2 Case-Control Studies biology.protein Female Antibody business Protein Binding
Zdroj:	Journal of immunoassayimmunochemistry. 34(2)
ISSN:	1532-4230
Popis:	Adiponectin (Adn) is a protein that circulates in the blood in several oligomeric forms, namely low-, medium-, and high-molecular-weight forms. Adn may serve as a risk factor for type 2 diabetes mellitus (T2DM). The aims of this work were (1) to produce monoclonal antibodies (MAbs) specific to different Adn oligomeric forms, (2) to design immunoassays suitable for measuring the Adn forms present in human blood, and (3) to investigate the changes in Adn forms that occur in patients with T2DM. Gel filtration, fluoroimmunoassays, and Western blotting were utilized as major techniques in this study. MAbs recognizing various oligomeric forms of Adn were obtained. Complexes between Adn and complement component C1q and between the low molecular weight form of Adn and albumin were described in human blood. A decrease in the total Adn and Adn-albumin complex levels in the blood of patients with T2DM and no difference in the levels of the Adn-C1q complex in comparison with healthy volunteers were demonstrated. An Adn94-Adn63 fluoroimmunoassay was selected as the technique that most accurately measured the mass ratio of Adn oligomers in blood samples, and an Adn214-Adn27 assay that measured the low-molecular-weight form of Adn only.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8c0ccf2e83375f9b941787c9d289680 https://pubmed.ncbi.nlm.nih.gov/23537302 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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