Copper caused reproductive endocrine disruption in zebrafish (Danio rerio)
| Autor: | Xinjin He, Lingtian Xie, Tianyu Wang, Panhong Wu, Jianjie Chen, Guodong Wang, Jinling Cao, Guo Wenjing |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
endocrine system Gonad Health Toxicology and Mutagenesis medicine.medical_treatment Danio Endocrine System Hypothalamic–pituitary–gonadal axis Endocrine Disruptors 010501 environmental sciences Aquatic Science Biology 01 natural sciences Andrology 03 medical and health sciences Reproductive biology medicine Animals Endocrine system Gonads Zebrafish 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Dose-Response Relationship Drug Reproduction Zebrafish Proteins biology.organism_classification Steroid hormone medicine.anatomical_structure Female Copper Water Pollutants Chemical Hormone |
| Zdroj: | Aquatic Toxicology. 211:124-136 |
| ISSN: | 0166-445X |
| DOI: | 10.1016/j.aquatox.2019.04.003 |
| Popis: | Cu in surface waters has been demonstrated to affect aquatic animals at ecologically relevant concentrations. However, its effects on reproductive endocrine system and the underlying toxicological mechanisms are largely unknown. In this study, zebrafish (Danio rerio) were exposed to 0, 10, 20, 40 μg/L of Cu for 30 days. Growth, gonad histopathology, the hormone levels and the transcriptional profiles of genes in the hypothalamic-pituitary-gonadal (HPG) axis in both sexes were examined. The results indicated that body weight was significantly reduced, the gonadal development was affected, and the levels of E2, T and 11-KT were remarkably disturbed in Cu-exposed fish. Moreover, the expression profiles of steroidogenesis-related genes in gonad (3βhsd, 17βhsd, cyp11a1, cyp17, cyp19a, lhr, fshr, hmgra and star) and in brains (ar, cyp19b, erα, er2β, lhβ, fshβ, gnrh2, gnrh3, gnrhr1, gnrh2 and gnrh4) displayed alterations after exposure to Cu. These results demonstrated that Cu could suppress the growth of zebrafish and significantly affect the reproductive biology in both sexes by damaging the structure of the gonads, altering the steroid hormone levels and the expressions of endocrine-related genes in HPG of zebrafish. This study suggests that Cu adversely affects the reproductive endocrine system in zebrafish and could pose a potential threat to fish populations inhabiting Cu-contaminated waters. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect