Superoxide dismutase-like activities of copper(II) complexes tested in serum
| Autor: | Elizabeth H. Griffith, Rajagopalan Sridhar, Joseph Roberts, Klaus R. Huber, Elmer L. Amma |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Xanthine Oxidase
Copper Sulfate Free Radicals Radical Inorganic chemistry Biophysics Biochemistry Xanthine Adduct law.invention Superoxide dismutase Cyclic N-Oxides chemistry.chemical_compound Structural Biology law Superoxides Hydroxides Animals Electron paramagnetic resonance Molecular Biology Oxidase test biology Spin trapping Superoxide Hydroxyl Radical Superoxide Dismutase Nitroblue Tetrazolium Electron Spin Resonance Spectroscopy chemistry Xanthines biology.protein Hydroxyl radical Cattle Spin Labels Oxidation-Reduction Copper Nuclear chemistry |
| Zdroj: | Biochimica et biophysica acta. 915(2) |
| ISSN: | 0006-3002 |
| Popis: | The superoxide dismutase-like activities of a series of coordination complexes of copper were evaluated and compared to the activities of bovine erythrocyte superoxide dismutase (superoxide: superoxide oxidoreductase, EC 1.15.1.1) in serum using the nitroblue tetrazolium chloride (NBT)-reduction assay and electron paramagnetic resonance (EPR) spectroscopy. A 40% inhibition was observed for the initial rate of the NBT reduction by superoxide dismutase in serum, but more than 40% inhibition was achieved with CuSO4, Cu(II)-dimethylglyoxime, Cu(II)-3,8-dimethyl-4,7-diazadeca-3,7-dienediamide, Cu2[N,N'-(2-(O-hydroxy-benzhydrylidene)amino)ethyl]2-1,2-ethane dia mine), Cu(II)-(diisopropylsalicylate)2, Cu(II)-(p-bromo-benzoate)2, Cu(II)-(nicotinate)2 and Cu(II)-(1,2-diamino-2-methylpropane)2. The electron paramagnetic resonance technique of spin trapping was used to detect the formation of superoxide (O2-.) and other free radicals in the xanthine-xanthine oxidase system under a variety of conditions. Addition of the spin trapping agent 5,5-dimethylpyrroline 1-oxide (DMPO) to the xanthine-xanthine oxidase system in fetal bovine serum produced the O2-.-spin adduct of DMPO (herein referred to as superoxide spin adduct, DMPO-OOH) as the well known short-lived nitroxyl whose characteristic EPR spectrum was recorded before its rapid decay to undetectable levels. The hydroxyl radical (HO.) adduct of the spin trap DMPO (herein referred to as DMPO-OH) was detected to a very small extent. When CuSO4, or the test complexes of copper, were added to the xanthine-xanthine oxidase system in serum containing the spin trap, the yield of DMPO-OOH was negligible. In addition to their superoxide dismutase-like activity, CuSO4 and the copper complexes also behaved as Fenton-type catalysts as seen by the accumulation of varying amounts of the hydroxyl spin adduct DMPO-OH. Both the Fenton-type catalysis and the superoxide dismutase-like action of these compounds were lost when a chelator such as EDTA was included in the xanthine-xanthine oxidase incubation mixture. Addition of superoxide dismutase instead of the copper compounds to this enzyme system abolished the formation of superoxide adduct DMPO-OOH, and no hydroxyl adduct DMPO-OH was detected. This effect of superoxide dismutase remained unaltered by EDTA. |
| Databáze: | OpenAIRE |
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