| Autor: |
Dae-Jin Yun, A. K. Kononowicz, Paul M. Hasegawa, Ray A. Bressan, Tracie K. Matsumoto, X. Niu, Y. Wu, A. A. Watad |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Plant cell reports. 17(4) |
| ISSN: |
1432-203X |
| Popis: |
We have obtained transgenic lily (Lilium longiflorum) plants after microprojectile bombardment, using the Biolistics PDS 1000/He system, of morphogenic calli derived from bulblet scales, followed by bialaphos selection. Parameters which gave the highest transient uidA expression were used: a bombardment pressure of 1100 psi, a target distance of 6 cm and a 48-h preculture on medium with 3% sucrose. A total of 1800 morphogenic calli were co-bombarded with plasmids containing either the uidA reporter or PAT selectable marker genes. After bombardment, the calli were exposed to 2 mg/l bialaphos. Only 72 of the shoot-forming calli (4%) survived. The 72 shoot clusters produced 342 shoots on elongation medium containing 0.5 mg/l bialaphos. Only 55 plantlets survived subsequent exposure to 2.0 mg/l bialaphos. PCR analysis indicated that 19 of these plantlets contained the PAT transgene. Southern analysis of 3 of the plants indicated that all contained the PAT gene. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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