Fluorescence diagnosis of endometriosis on the chorioallantoic membrane using 5-aminolaevulinic acid
| Autor: | W. Böhm, A. Rück, Ch. Berg, Klaus Diedrich, E. Malik, K. Kunzi-Rapp, A. Meyhöfer-Malik |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Erythrocytes Time Factors Necrosis Transplantation Heterologous Fimbria Endometriosis Neovascularization Physiologic Chick Embryo Biology Endometrium Fluorescence Andrology Peritoneum Allantois medicine Animals Humans Incubation Rehabilitation Obstetrics and Gynecology Aminolevulinic Acid medicine.disease Transplantation Chorioallantoic membrane medicine.anatomical_structure Reproductive Medicine Female medicine.symptom |
| Zdroj: | Human Reproduction. 15:584-588 |
| ISSN: | 1460-2350 0268-1161 |
| Popis: | The chorioallantoic membrane (CAM) is a useful model for the fluorescence diagnosis of experimentally induced endometriosis. In our experimental setup 75.7% of the histologically examined tissue preparations were viable and only 24.3% showed signs of necrosis on the CAM after various periods of incubation. Best results were obtained when grafting to the CAM was performed between days 7 and 9 and when implants were left on the CAM for 3-5 days (P < 0.05). We were able to demonstrate that 5-aminolaevulinic acid (ALA) is stored selectively in ectopic endometrium. The subsequent fluorescence of the endometrium shows a rapid increase that reaches a peak after 10-14 h which can be clearly differentiated from the weaker fluorescence of grafted normal peritoneum and fimbriae (P < 0.01). |
| Databáze: | OpenAIRE |
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