Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

Autor:	Chiara Luberto, Ling Li, Eric Brouzes, Phenix-Lan Quan, Jinzhu Yu, Sitapriya Moorthi, Michael W. Schuster, Ki Oh, Helmut H. Strey
Rok vydání:	2020
Předmět:	Intracellular signaling pathway Cell Fusion Proteins bcr-abl Biomedical Engineering Bioengineering Biochemistry Article Flow cytometry 03 medical and health sciences Bcr abl1 0302 clinical medicine Leukemia Myelogenous Chronic BCR-ABL Positive medicine Humans Protein phosphorylation Phosphorylation Protein Kinase Inhibitors 030304 developmental biology chemistry.chemical_classification 0303 health sciences medicine.diagnostic_test General Chemistry Flow Cytometry Cell biology Enzyme medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Signal amplification Cytometry Signal Transduction
Zdroj:	Lab Chip
ISSN:	1473-0189 1473-0197
DOI:	10.1039/c9lc01213c
Popis:	We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8b9b54ba118cc3dd0183b49d0045d36 https://doi.org/10.1039/c9lc01213c Zobrazit plný text záznamu