A novel experimental model of acute respiratory distress syndrome in pig
| Autor: | Otomar Kittnar, I Vítková, Mikulas Mlcek, Michal Otahal |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
ARDS
Physiology Swine Sus scrofa Hemodynamics Bronchoalveolar Lavage 03 medical and health sciences 0302 clinical medicine medicine Animals Ketamine Lung Respiratory Distress Syndrome business.industry 030208 emergency & critical care medicine General Medicine medicine.disease Disease Models Animal Blood pressure 030228 respiratory system Anesthesia Breathing Midazolam Premedication Female business Propofol medicine.drug |
| Zdroj: | ResearcherID Scopus-Elsevier |
| ISSN: | 1802-9973 |
| Popis: | Acute respiratory distress syndrome (ARDS) is severe medical condition occurring in critically ill patients and with mortality of 33-52 % is one of the leading causes of death in critically ill patients. To better understand pathophysiology of ARDS and to verify novel therapeutical approaches a reliable animal model is needed. Therefore we have developed modified lavage model of ARDS in the pig. After premedication (ketamine and midazolam) 35 healthy pigs were anesthetized (propofol, midazolam, morphin, pipecuronium) and orotracheally intubated and ventilated. Primary ARDS was induced by repeated cycles of lung lavage with a detergent Triton X100 diluted in saline (0.03 %) heated to 37 °C preceded by pre-oxygenation with 100 % O2. Single cycle included two subsequent lavages followed by detergent suction. Each cycle was followed by hemodynamic and ventilation stabilization for approx. 15 min, with eventual administration of vasopressors according to an arterial blood pressure. The lavage procedure was repeated until the paO2/FiO2 index after stabilization remained below 100 at PEEP 5 cm H2O. In 33 pigs we have achieved the desired degree of severe ARDS (PaO2/FiO2 |
| Databáze: | OpenAIRE |
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