Shotgun Proteomics Analysis of Saliva and Salivary Gland Tissue from the Common Octopus Octopus vulgaris
| Autor: | Pierre Faou, Jan M. Strugnell, Jia Zhang, Ira Cooke, Alvaro Roura Labiaga, Legana C. H. W. Fingerhut |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Proteomics 0301 basic medicine Saliva Proteome Neurotoxins Octopodiformes Mollusk Venoms Biochemistry Salivary Glands Cephalopod Transcriptome 03 medical and health sciences Venomics medicine Animals Transcriptomics Shotgun proteomics Phylogeny Proteogenomics Salivary gland biology tTandem mass spectrometry Common octopus Gene Expression Regulation Developmental Molecular Sequence Annotation Octopus vulgaris General Chemistry Venom Protein biology.organism_classification Venom Gene Ontology 030104 developmental biology medicine.anatomical_structure Larva Female Serine Proteases Toxin |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/acs.jproteome.8b00525 |
| Popis: | 11 pages, 3 figures The salivary apparatus of the common octopus (Octopus vulgaris) has been the subject of biochemical study for over a century. A combination of bioassays, behavioral studies and molecular analysis on O. vulgaris and related species suggests that its proteome should contain a mixture of highly potent neurotoxins and degradative proteins. However, a lack of genomic and transcriptomic data has meant that the amino acid sequences of these proteins remain almost entirely unknown. To address this, we assembled the posterior salivary gland transcriptome of O. vulgaris and combined it with high resolution mass spectrometry data from the posterior and anterior salivary glands of two adults, the posterior salivary glands of six paralarvae and the saliva from a single adult. We identified a total of 2810 protein groups from across this range of salivary tissues and age classes, including 84 with homology to known venom protein families. Additionally, we found 21 short secreted cysteine rich protein groups of which 12 were specific to cephalopods. By combining protein expression data with phylogenetic analysis we demonstrate that serine proteases expanded dramatically within the cephalopod lineage and that cephalopod specific proteins are strongly associated with the salivary apparatus This study was funded by a La Trobe University Understanding Disease Research Focus Area small project grant |
| Databáze: | OpenAIRE |
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