Hydrogen Peroxide and Nitric Oxide Crosstalk Mediates Brassinosteroids Induced Cold Stress Tolerance in Medicago truncatula

Autor: Tong Zhu, Da-Wei Zhang, Shi-Shuai Luo, Lijuan Zou, Wen-Rong Tan, Muhammad Arfan, Hong-Hui Lin
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Photosystem II
Acclimatization
PSII activity
Medicago truncatula
01 natural sciences
lcsh:Chemistry
chemistry.chemical_compound
Hydrogen peroxide
lcsh:QH301-705.5
Spectroscopy
Plant Proteins
Photosystem
biology
Imidazoles
Thiourea
food and beverages
Free Radical Scavengers
General Medicine
Computer Science Applications
Cell biology
cold stress tolerance
Oxidoreductases
Signal Transduction
Alternative oxidase
hydrogen peroxide
Article
Catalysis
Nitric oxide
Cyclic N-Oxides
Mitochondrial Proteins
Inorganic Chemistry
03 medical and health sciences
nitric oxide
Physical and Theoretical Chemistry
Molecular Biology
Brassinolide
Abiotic stress
Cold-Shock Response
Organic Chemistry
fungi
biology.organism_classification
030104 developmental biology
brassinosteroids
chemistry
lcsh:Biology (General)
lcsh:QD1-999
AOX capacity
010606 plant biology & botany
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 1, p 144 (2019)
International Journal of Molecular Sciences
Volume 20
Issue 1
ISSN: 1422-0067
Popis: Brassinosteroids (BRs) play pivotal roles in modulating plant growth, development, and stress responses. In this study, a Medicago truncatula plant pretreated with brassinolide (BL, the most active BR), enhanced cold stress tolerance by regulating the expression of several cold-related genes and antioxidant enzymes activities. Previous studies reported that hydrogen peroxide (H2O2) and nitric oxide (NO) are involved during environmental stress conditions. However, how these two signaling molecules interact with each other in BRs-induced abiotic stress tolerance remain largely unclear. BL-pretreatment induced, while brassinazole (BRZ, a specific inhibitor of BRs biosynthesis) reduced H2O2 and NO production. Further, application of dimethylthiourea (DMTU, a H2O2 and OH&minus
scavenger) blocked BRs-induced NO production, but BRs-induced H2O2 generation was not sensitive to 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO, a scavenger of NO). Moreover, pretreatment with DMTU and PTIO decreased BL-induced mitochondrial alternative oxidase (AOX) and the photosystem capacity. However, pretreatment with PTIO was found to be more effective than DMTU in reducing BRs-induced increases in Valt, Vt, and MtAOX1 gene expression. Similarly, BRs-induced photosystem II efficiency was found in NO dependent manner than H2O2. Finally, we conclude that H2O2 was involved in NO generation, whereas NO was found to be crucial in BRs-induced AOX capacity, which further contributed to the protection of the photosystem under cold stress conditions in Medicago truncatula.
Databáze: OpenAIRE
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