Case Report of Rat True Hermaphroditism: Colocalization of Oocytes and Granulosa and Sertoli Cells in the Germinal Cord
| Autor: | Kiyonori Kai, Kazuhisa Furuhama, Kazumi Shiraiwa, Astushi Watanabe, Hironobu Sasano, Norihiro Satoh |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
endocrine system Pathology medicine.medical_specialty 040301 veterinary sciences Disorders of Sex Development Biology Toxicology 030226 pharmacology & pharmacy Pathology and Forensic Medicine 0403 veterinary science Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine medicine True hermaphroditism Animals Molecular Biology Granulosa Cells Sertoli Cells Ovotestis urogenital system Theca interna 04 agricultural and veterinary sciences Cell Biology Left Testis medicine.disease Sertoli cell Rats Microscopy Electron medicine.anatomical_structure Theca Oocytes Basal lamina Female Spermatogenesis |
| Zdroj: | Toxicologic Pathology. 31:290-294 |
| ISSN: | 1533-1601 0192-6233 |
| DOI: | 10.1080/01926230309755 |
| Popis: | We describe a case of rat hermaphroditism with bilateral ovotestes. In a 7-week-old apparently male Sprague—Dawley rat, both testes were relatively small, and the right testis with a faint protrusion was somewhat round and small as compared with the left testis. Microscopically, the testes contained ovarian tissues within their tunica albugineas in conjunction with spermatogenesis in the seminiferous tubules. As bilateral changes, oocytes surrounded by granulosa-like cells were present in the seminiferous tubule-like germinal cord. Granulosa-like and Sertoli-like cells were layered together on the basal lamina, and theca interna-like cells were occasionally observed around the basal lamina. As unilateral changes, cystic dilatation of the germinal cords with eosinophilic fluid was seen in the lumen, and the theca interna-like cells appeared to be vacuolated. Immunohistochemically, the granulosa-like and Sertoli-like cells showed positive reactions for 3 β-hydroxysteroid dehydrogenase (3 β-HSD) and vimentin, respectively. Theca interna-like cells reacted positively to both 3 β-HSD and cytochrome P-450 17 α -hydroxylase. Ultrastructurally, the granulosa, Sertoli, and theca interna cells were also identified in the ovarian tissue. From these morphological characteristics, the male rat with bilateral ovotestes was diagnosed as true hermaphroditism. |
| Databáze: | OpenAIRE |
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