Profiling of proteins secreted in the bovine oviduct reveals diverse functions of this luminal microenvironment

Autor: Darren Weber, Vimal Selvaraj, Brett S. Phinney, Viju V. Pillai
Přispěvatelé: Kues, Wilfried A
Rok vydání: 2017
Předmět:
0301 basic medicine
Proteomics
Embryology
Physiology
lcsh:Medicine
Gene Expression
Biochemistry
Extracellular matrix
0302 clinical medicine
Endocrinology
Animal Cells
Medicine and Health Sciences
lcsh:Science
Extracellular Matrix Proteins
030219 obstetrics & reproductive medicine
Multidisciplinary
Embryo
Proteases
Cell biology
Enzymes
medicine.anatomical_structure
Oviduct
Gamete
Intercellular Signaling Peptides and Proteins
Female
Cellular Types
Biotechnology
Research Article
animal structures
General Science & Technology
1.1 Normal biological development and functioning
Lipoproteins
Biology
03 medical and health sciences
Underpinning research
Growth Factors
medicine
Animals
Secretion
Shotgun proteomics
Fallopian Tubes
Endocrine Physiology
Contraception/Reproduction
lcsh:R
Embryos
Biology and Life Sciences
Proteins
Epithelial Cells
Molecular Sequence Annotation
Cell Biology
Sperm
030104 developmental biology
Secretory protein
Gene Ontology
Germ Cells
Apolipoproteins
Fertilization
Enzymology
lcsh:Q
Cattle
Physiological Processes
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 11, p e0188105 (2017)
PloS one, vol 12, iss 11
ISSN: 1932-6203
Popis: The oviductal microenvironment is a site for key events that involve gamete maturation, fertilization and early embryo development. Secretions into the oviductal lumen by either the lining epithelium or by transudation of plasma constituents are known to contain elements conducive for reproductive success. Although previous studies have identified some of these factors involved in reproduction, knowledge of secreted proteins in the oviductal fluid remains rudimentary with limited definition of function even in extensively studied species like cattle. In this study, we used a shotgun proteomics approach followed by bioinformatics sequence prediction to identify secreted proteins present in the bovine oviductal fluid (ex vivo) and secretions from the bovine oviductal epithelial cells (in vitro). From a total of 2087 proteins identified, 266 proteins could be classified as secreted, 109 (41%) of which were common for both in vivo and in vitro conditions. Pathway analysis indicated different classes of proteins that included growth factors, metabolic regulators, immune modulators, enzymes, and extracellular matrix components. Functional analysis revealed mechanisms in the oviductal lumen linked to immune homeostasis, gamete maturation, fertilization and early embryo development. These results point to several novel components that work together with known elements mediating functional homeostasis, and highlight the diversity of machinery associated with oviductal physiology and early events in cattle fertility.
Databáze: OpenAIRE
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