Treatment with the MAPK kinase inhibitor U0126 during the first two hours of in vitro maturation improves bovine oocyte developmental competence

Autor:	T Shinohara, Noritoshi Kawate, Toshio Inaba, Nobutada Sakagami, Masahiro Takahashi, Hiromichi Tamada, Y. Ohta
Rok vydání:	2017
Předmět:	0301 basic medicine MAPK/ERK pathway medicine.medical_specialty Connexin Cell Communication Fertilization in Vitro Biology 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine Nitriles Butadienes medicine Animals Blastocyst Mitogen-Activated Protein Kinase Kinases Cumulus Cells 030219 obstetrics & reproductive medicine Mapk kinase Gap junction Gap Junctions Oocyte Immunohistochemistry In Vitro Oocyte Maturation Techniques In vitro maturation 030104 developmental biology medicine.anatomical_structure Connexin 43 embryonic structures Oocytes Cattle Female Animal Science and Zoology sense organs hormones hormone substitutes and hormone antagonists Biotechnology
Zdroj:	Reproduction in Domestic Animals. 53:270-273
ISSN:	0936-6768
Popis:	This study examined the effects of treatment with U0126, which inhibits MAPK by inhibiting MAPK kinase, during the first 2 hr of in vitro maturation on bovine developmental competence and on gap junction (GAPJ) communication between the oocyte and cumulus cells. The percentage of oocytes developing to the blastocyst stage in the group treated with 5 μM U0126 (28%) was significantly higher than that in controls (15%, p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8b2a66c6a34aeda004a39069a02cca5 https://doi.org/10.1111/rda.13096 Zobrazit plný text záznamu Plný text