In-Vitro Antioxidant, Hypoglycemic Activity, and Identification of Bioactive Compounds in Phenol-Rich Extract from the Marine Red Algae Gracilaria edulis (Gmelin) Silva
| Autor: | P. Ranasinghe, Thilina Lakmini Gunathilaka, L Dinithi C Peiris, KW Samarakoon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
glucose diffusion
Antioxidant DPPH medicine.medical_treatment Ethyl acetate Pharmaceutical Science Red algae antiglycation 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound lcsh:Organic chemistry Drug Discovery medicine Phenol Food science fractionation Physical and Theoretical Chemistry 030304 developmental biology methanol extract 0303 health sciences Chloroform ABTS biology 010405 organic chemistry Organic Chemistry biology.organism_classification 0104 chemical sciences α-amylase chemistry Chemistry (miscellaneous) Molecular Medicine Ferric Gracillaria edulis α-glucosidase medicine.drug |
| Zdroj: | Molecules Volume 24 Issue 20 Molecules, Vol 24, Iss 20, p 3708 (2019) |
| ISSN: | 1420-3049 |
| Popis: | Obesity and diabetes are major metabolic disorders which are prevalent worldwide. Algae has played an important role in managing these disorders. In this study, Gracilaria edulis, a marine red algae, was investigated for antioxidant and hypoglycemic potential using in vitro models. De-polysaccharide methanol extract of G. edulis was sequentially partitioned with hexane, chloroform, ethyl acetate, and antioxidants, and hypoglycemic potentials were evaluated using multiple methods. High antioxidant potential was observed in the ethyl acetate fraction in terms of ferric reducing antioxidant power, iron chelating, and DPPH and ABTS radical scavenging activities, while the crude methanol extract exhibited potent oxygen radical-absorbance capacity. Potent &alpha amylase inhibitory activity was observed in the ethyl acetate fraction, while the ethyl acetate fraction was effective against &alpha glucosidase inhibition. Glucose diffusion was inhibited by the ethyl acetate fraction at 180 min, and the highest antiglycation activity was observed in both chloroform and ethyl acetate fractions. Additionally, gas chromatography-mass spectrometry analysis of the ethyl acetate fraction revealed the presence of several potent anti-diabetic compounds. In conclusion, G. edulis exhibited promising antidiabetic potential via multiple mechanisms. The ethyl acetate fraction exhibited the strongest hypoglycemic and antiglycation potential among the four fractions, and hence the isolation of active compounds is required to develop leads for new drugs to treat diabetes. |
| Databáze: | OpenAIRE |
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