In vitro biosynthesis of Ag, Au and Te-containing nanostructures by Exiguobacterium cell-free extracts
| Autor: | Claudia M. Muñoz-Villagrán, Javier Orizola, Felipe A. Arenas, Claudio C. Vásquez, Esteban Vargas, Mirtha Ríos-Silva |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cell Extracts
Silver Nanostructure Microorganism Metal ions in aqueous solution lcsh:Biotechnology chemistry.chemical_element Metal Nanoparticles Microbial Sensitivity Tests Biology Metal 03 medical and health sciences Bioremediation Exiguobacterium Exiguobacterium acetylicum lcsh:TP248.13-248.65 Anaerobiosis 030304 developmental biology Reduction 0303 health sciences 030306 microbiology Temperature biology.organism_classification Aerobiosis Anti-Bacterial Agents Biodegradation Environmental chemistry visual_art visual_art.visual_art_medium Metal(loid) Gold Tellurium Bacteria Biotechnology Nuclear chemistry Research Article |
| Zdroj: | BMC Biotechnology, Vol 20, Iss 1, Pp 1-12 (2020) BMC Biotechnology |
| ISSN: | 1472-6750 |
| Popis: | Background The bacterial genus Exiguobacterium includes several species that inhabit environments with a wide range of temperature, salinity, and pH. This is why the microorganisms from this genus are known generically as polyextremophiles. Several environmental isolates have been explored and characterized for enzyme production as well as for bioremediation purposes. In this line, toxic metal(loid) reduction by these microorganisms represents an approach to decontaminate soluble metal ions via their transformation into less toxic, insoluble derivatives. Microbial-mediated metal(loid) reduction frequently results in the synthesis of nanoscale structures—nanostructures (NS) —. Thus, microorganisms could be used as an ecofriendly way to get NS. Results We analyzed the tolerance of Exiguobacterium acetylicum MF03, E. aurantiacum MF06, and E. profundum MF08 to Silver (I), gold (III), and tellurium (IV) compounds. Specifically, we explored the ability of cell-free extracts from these bacteria to reduce these toxicants and synthesize NS in vitro, both in the presence or absence of oxygen. All isolates exhibited higher tolerance to these toxicants in anaerobiosis. While in the absence of oxygen they showed high tellurite- and silver-reducing activity at pH 9.0, whereas AuCl4− which was reduced at pH 7.0 in both conditions. Given these results, cell-free extracts were used to synthesize NS containing silver, gold or tellurium, characterizing their size, morphology and chemical composition. Silver and tellurium NS exhibited smaller size under anaerobiosis and their morphology was circular (silver NS), starred (tellurium NS) or amorphous (gold NS). Conclusions This nanostructure-synthesizing ability makes these isolates interesting candidates to get NS with biotechnological potential. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |