Generation and characterization of a functional nanobody against the vascular endothelial growth factor receptor-2; angiogenesis cell receptor

Autor:	Mahdi Behdani, Nader Asadzadeh, Serge Muyldermans, Keyhan Azadmanesh, Gholamreza Hassanzadeh-Ghassabeh, Steve Schoonooghe, Alireza Khabiri, Mahdi Habibi Anbouhi, Morteza Karimipour, Hossein Khanahmad, Sirous Zeinali
Přispěvatelé:	Cellular and Molecular Immunology, Department of Bio-engineering Sciences, Biotechnology Research Center, Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Department of Animal Breeding and Genetics, Animal Science Research Institute of IRAN, Réseau International des Instituts Pasteur (RIIP), Vrije Universiteit Brussel (VUB)
Jazyk:	angličtina
Rok vydání:	2012
Předmět:	Male MESH: Signal Transduction MESH: Sequence Homology Amino Acid Angiogenesis Angiogenesis Inhibitors MESH: Flow Cytometry MESH: Amino Acid Sequence Neovascularization chemistry.chemical_compound angiogenesis 0302 clinical medicine Antibody Specificity MESH: Immune Sera MESH: Animals MESH: Human Umbilical Vein Endothelial Cells Receptor Cells Cultured MESH: Angiogenesis Inhibitors MESH: Single-Chain Antibodies 0303 health sciences MESH: Kinetics respiratory system Flow Cytometry MESH: Surface Plasmon Resonance 3. Good health Cell biology Vascular endothelial growth factor VEGFR2 MESH: HEK293 Cells 030220 oncology & carcinogenesis MESH: Camelids New World cardiovascular system Electrophoresis Polyacrylamide Gel Signal transduction medicine.symptom Camelids New World MESH: Neovascularization Physiologic Protein Binding Signal Transduction MESH: Cells Cultured circulatory and respiratory physiology Molecular Sequence Data Immunology Neovascularization Physiologic Biology 03 medical and health sciences Human Umbilical Vein Endothelial Cells medicine Animals Humans MESH: Protein Binding Amino Acid Sequence MESH: Antibody Specificity Molecular Biology 030304 developmental biology [SDV.GEN]Life Sciences [q-bio]/Genetics MESH: Humans MESH: Molecular Sequence Data Sequence Homology Amino Acid MESH: Vascular Endothelial Growth Factor Receptor-2 Immune Sera HEK 293 cells Kinase insert domain receptor Surface Plasmon Resonance Vascular Endothelial Growth Factor Receptor-2 Molecular biology MESH: Male Kinetics HEK293 Cells chemistry Cell culture Nanobody Single-Chain Antibodies MESH: Electrophoresis Polyacrylamide Gel
Zdroj:	Molecular Immunology Molecular Immunology, Elsevier, 2012, 50 (1-2), pp.35-41. ⟨10.1016/j.molimm.2011.11.013⟩ Vrije Universiteit Brussel
ISSN:	0161-5890
DOI:	10.1016/j.molimm.2011.11.013⟩
Popis:	International audience; Vascular endothelial growth factor receptor-2 (VEGFR2) is an important tumor-associated receptor and blockade of the VEGF receptor signaling can lead to the inhibition of neovascularization and tumor metastasis. Nanobodies are the smallest intact antigen binding fragments derived from heavy chain-only antibodies occurring in camelids. Here, we describe the identification of a VEGFR2-specific Nanobody, named 3VGR19, from dromedaries immunized with a cell line expressing high levels of VEGFR2. We demonstrate by FACS, that 3VGR19 Nanobody specifically binds VEGFR2 on the surface of 293KDR and HUVECs cells. Furthermore, the 3VGR19 Nanobody potently inhibits formation of capillary-like structures. These data show the potential of Nanobodies for the blockade of VEGFR2 signaling and provide a basis for the development of novel cancer therapeutics.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8ad5ff225a948ca56e5e3a955a1b7f2 https://biblio.vub.ac.be/vubir/generation-and-characterization-of-a-functional-nanobody-against-the-vascular-endothelial-growth-factor-receptor2-angiogenesis-cell-receptor(52744170-4794-471b-9ba4-a61f18f87c3d).html Zobrazit plný text záznamu Full Text from ScienceDirect