Determination of Toxic Activities inBothropsspp. Snake Venoms Using Animal-Free Approaches: Correlation BetweenIn VitroVersusIn VivoAssays
| Autor: | Letícia Lopes de Souza, Ana Flor-Sá, Carlos Chávez-Olórtegui, Clara Guerra-Duarte, Francisco Santos Schneider, Evanguedes Kalapothakis, Stephanie Stransky |
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| Rok vydání: | 2015 |
| Předmět: |
Necrosis
Cell Survival Antivenom Hyaluronoglucosaminidase In Vitro Techniques Biology Pharmacology Animal Testing Alternatives L-Amino Acid Oxidase Toxicology Cell Line Mice In vivo Hyaluronidase Crotalid Venoms medicine Animals Humans Bioassay Antigens Human Platelet Bothrops Cytotoxicity Antivenins In vitro toxicology biology.organism_classification Proteolysis Immunology medicine.symptom medicine.drug |
| Zdroj: | Toxicological Sciences. 147:458-465 |
| ISSN: | 1096-0929 1096-6080 |
| DOI: | 10.1093/toxsci/kfv140 |
| Popis: | The main purpose of this study is to investigate the in vitro toxic effects of 5 Bothrops spp. snake venoms, which are part of the antigenic mixture used for the production of Brazilian antivenom, and evaluate their correlation with the in vivo toxic activities of Bothrops spp. venoms. The correlation analysis could be helpful for the replacement of living animals experimentation for in vitro bioassay. Cytotoxicity, L-amino acid oxidase (LAAO), proteolitic (serine and metalloproteinase), hyaluronidase (Hyal), and phospholipase A2 (PLA2) activities were estimated and the correlation coefficient was determined for each activity in relation to lethality, edema, hemorrhage and necrosis induced in live animals by B. jararaca, B. alternatus, B. jararacussu, B. neuwiedi, and B. moojeni venoms. The lethal activity in mice was highly related to Hyal activity (r = 0.94, p < .05), edema related to PLA2 activity (r = 0.94, p < .05), whereas the necrotizing activity showed high correlation with LAAO activity (r = 0.83, p < .05). A very significant correlation between in vitro cytotoxicity and LAAO activities was also observed (r = 0.97, p < .05). |
| Databáze: | OpenAIRE |
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