Short-term spheroid culture of primary colorectal cancer cells as an in vitro model for personalizing cancer medicine

Autor: Per Ibsen, Ben Vainer, Maria Jeppesen, Jacob Thastrup, Ole Thastrup, Anders Glenthøj, Lars N. Jorgensen, Grith Hagel, Henrik Harling
Rok vydání: 2017
Předmět:
0301 basic medicine
Oncology
Cellular pathology
Colorectal cancer
Cancer Treatment
lcsh:Medicine
Immunostaining
Keratin-20
Epithelium
0302 clinical medicine
Carcinoembryonic antigen
Animal Cells
Adenocarcinomas
Epithelial Cells/pathology
Medicine and Health Sciences
Tumor Cells
Cultured
Precision Medicine
lcsh:Science
Connective Tissue Cells
Staining
Adenocarcinoma/pathology
Multidisciplinary
biology
Cell Staining
Neoplasm Proteins
Connective Tissue
Spheroids
Cellular/pathology
030220 oncology & carcinogenesis
embryonic structures
Adenocarcinoma
Cellular Types
Anatomy
Colorectal Neoplasms
Research Article
medicine.medical_specialty
Cell Survival
Keratin-20/metabolism
Research and Analysis Methods
Carcinomas
Models
Biological
03 medical and health sciences
Cytokeratin
Spheroids
Cellular
Internal medicine
medicine
Humans
Neoplasm Proteins/metabolism
Cell Proliferation
Colorectal Cancer
Colorectal Neoplasms/pathology
business.industry
lcsh:R
Spheroid
Cancers and Neoplasms
Biology and Life Sciences
Cancer
Epithelial Cells
Cell Biology
Fibroblasts
medicine.disease
Fibroblasts/pathology
Nuclear Staining
Biological Tissue
030104 developmental biology
Specimen Preparation and Treatment
Cancer cell
biology.protein
lcsh:Q
business
Zdroj: PLoS ONE, Vol 12, Iss 9, p e0183074 (2017)
Jeppesen, M, Hagel, G, Glenthoj, A, Vainer, B, Ibsen, P, Harling, H, Thastrup, O, Jørgensen, L N & Thastrup, J 2017, ' Short-term spheroid culture of primary colorectal cancer cells as an in vitro model for personalizing cancer medicine ', PLOS ONE, vol. 12, no. 9, e0183074 . https://doi.org/10.1371/journal.pone.0183074
PLoS ONE
ISSN: 1932-6203
Popis: Chemotherapy treatment of cancer remains a challenge due to the molecular and functional heterogeneity displayed by tumours originating from the same cell type. The pronounced heterogeneity makes it difficult for oncologists to devise an effective therapeutic strategy for the patient. One approach for increasing treatment efficacy is to test the chemosensitivity of cancer cells obtained from the patient's tumour. 3D culture represents a promising method for modelling patient tumours in vitro. The aim of this study was therefore to evaluate how closely short-term spheroid cultures of primary colorectal cancer cells resemble the original tumour. Colorectal cancer cells were isolated from human tumour tissue and cultured as spheroids. Spheroid cultures were established with a high success rate and remained viable for at least 10 days. The spheroids exhibited significant growth over a period of 7 days and no difference in growth rate was observed for spheroids of different sizes. Comparison of spheroids with the original tumour revealed that spheroid culture generally preserved adenocarcinoma histology and expression patterns of cytokeratin 20 and carcinoembryonic antigen. Interestingly, spheroids had a tendency to resemble tumour protein expression more closely after 10 days of culture compared to 3 days. Chemosensitivity screening using spheroids from five patients demonstrated individual response profiles. This indicates that the spheroids maintained patient-to-patient differences in sensitivity towards the drugs and combinations most commonly used for treatment of colorectal cancer. In summary, short-term spheroid culture of primary colorectal adenocarcinoma cells represents a promising in vitro model for use in personalized medicine.
Databáze: OpenAIRE
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