Requirement for Activin a and Transforming Growth Factor--β1 Pro-Regions in Homodimer Assembly
| Autor: | Anthony J. Mason, Alane M. Gray |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Macromolecular Substances
Immunoprecipitation medicine.medical_treatment Molecular Sequence Data Protein Sorting Signals Biology Transfection medicine Humans Inhibins Secretion Amino Acid Sequence Peptide sequence Cells Cultured Multidisciplinary Growth factor Genetic Complementation Test Transforming growth factor beta Activins Complementation Biochemistry Transforming Growth Factors biology.protein Protein Processing Post-Translational ACVR2B Transforming growth factor |
| Zdroj: | Science. 247:1328-1330 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.2315700 |
| Popis: | Many proteins are initially synthesized as part of a large precursor. The role of the pro-region in the biosynthesis of transforming growth factor--beta 1 (TGF-beta 1) and activin A, two structurally related disulfide-linked homodimers synthesized as large precursors, was studied. Vectors that expressed either the pro-region or the mature regions of these molecules were used in complementation experiments, only when the pro-region was coexpressed with the mature region did intracellular dimerization and secretion of biologically active homodimers occur. The pro-regions of activin A and TGF-beta 1, therefore, aid the folding, disulfide bond formation, and export of their respective homodimers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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