Expression and modulation of the Lewis x antigen (CD15) on the T cell line Molt-4
| Autor: | Kai Schulze-Forster, H. Rainer Maurer |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Phorbol ester
Leukemia T-Cell T-Lymphocytes T cell Biophysics Lewis X Antigen Cell Count Biology Thymus Extracts Biochemistry Fluorescence Flow cytometry Adjuvants Immunologic Antigen Structural Biology Tumor Cells Cultured Genetics medicine Humans Cytotoxic T cell CD15 expression Antigen-presenting cell Molecular Biology medicine.diagnostic_test Cell adhesion molecule Thymic peptide Cell Biology Flow Cytometry Molt-4 cell Molecular biology Gene Expression Regulation Neoplastic medicine.anatomical_structure Tetradecanoylphorbol Acetate Antigens Surface Cytokines |
| Zdroj: | FEBS Letters. 415:25-28 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(97)01084-3 |
| Popis: | The T cell lines Molt-4 and H9 exhibited a characteristic distribution of the cell adhesion molecule Lewis x (CD15, lacto-N-fucopentanose III) showing an unusually broad peak by flow cytometry ranging from cells without CD15 to cells with high expression. The cytokines IL-1, IL-2, IFN-beta, IFN-gamma, and TNF-alpha, known to activate T cells, did not affect CD15 expression. However, phorbol myristate acetate and the thymic peptide extract Thymex-L were able to enhance both the number of CD15-positive cells and the median fluorescence. The effects of both inducers were dose- and time-dependent. An additive or synergistic effect was not seen. These data suggest that phorbol esters and distinct thymic peptides can modulate the expression of the cell surface antigen CD15. |
| Databáze: | OpenAIRE |
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