Designing Zn(II) and Cu(II) derivatives as probes for in vitro fluorescence imaging
| Autor: | Tzveta D. Pokrovska, Timothy D. Conry, Helen M. Betts, Jonathan R. Dilworth, John E. Warren, Franklin I. Aigbirhio, Sofia I. Pascu, Martin Christlieb, Philip A. Waghorn, Grant C. Churchill |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Magnetic Resonance Spectroscopy biology Inorganic chemistry chemistry.chemical_element Zinc biology.organism_classification Crystallography X-Ray Fluorescence Inorganic Chemistry HeLa Transmetalation chemistry.chemical_compound chemistry Microscopy Fluorescence Cell Line Tumor Molecular Probes Microscopy Humans Semicarbazone Single crystal Copper Nuclear chemistry |
| Zdroj: | Dalton transactions (Cambridge, England : 2003). (43) |
| ISSN: | 1477-9226 |
| Popis: | New M(II) bis(thiosemicarbazonato) complexes (M = Ni(II), Cu(II) and Zn(II)) featuring allyl groups at the exocyclic nitrogens have been synthesised. The complexes were characterised in solution by spectroscopic methods and their solid state structures determined by single crystal X-ray diffraction using synchrotron radiation. The Zn(II) complex was found to be intrinsically fluorescent and soluble in biocompatible media. The uptake of this Zn(II) complex in HeLa, MCF-7 and IGROV cancer cells was monitored by fluorescence microscopies (epi- and confocal fluorescence imaging). The radiolabelling to (64)Cu(II) bis(thiosemicarbazonato) complex was performed cleanly by transmetallation from the corresponding Zn(II) species using (64)Cu(OAc)(2). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|