IGF-1 induction by acylated steryl β-glucosides found in a pre-germinated brown rice diet reduces oxidative stress in streptozotocin-induced diabetes

Autor: Robert K. Yu, Ying Ying Tsai, Yasuhide Okuhara, Shota Nonaka, Seigo Usuki, Mitsuo Kise, Keiko Morikawa
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Blood Glucose
Male
Anatomy and Physiology
Mitochondrial Diseases
Diabetic neuropathy
Acylation
medicine.medical_treatment
lcsh:Medicine
Apoptosis
medicine.disease_cause
Pentose Phosphate Pathway
Endocrinology
Glucosides
Insulin
Insulin-Like Growth Factor I
lcsh:Science
Insulin-like Growth Factor
Multidisciplinary
medicine.anatomical_structure
Medicine
Brown rice
Pancreas
Glycolysis
Research Article
medicine.drug
medicine.medical_specialty
Cell Survival
Endocrine System
Germination
Glucosephosphate Dehydrogenase
Carbohydrate metabolism
Biology
Models
Biological
Diabetes Mellitus
Experimental
Internal medicine
Diabetes mellitus
medicine
Animals
RNA
Messenger
Rats
Wistar
Clinical Genetics
Diabetic Endocrinology
Endocrine Physiology
Body Weight
lcsh:R
Oryza
Diabetes Mellitus Type 1
Diabetes Mellitus Type 2
medicine.disease
Streptozotocin
Culture Media
Diet
Rats
Oxidative Stress
Diabetes Mellitus and Deafness
Glucose
Gene Expression Regulation
lcsh:Q
Reactive Oxygen Species
Oxidative stress
Zdroj: PLoS ONE, Vol 6, Iss 12, p e28693 (2011)
PLoS ONE
ISSN: 1932-6203
Popis: BACKGROUND: The pathology of diabetic neuropathy involves oxidative stress on pancreatic β-cells, and is related to decreased levels of Insulin-like growth factor 1 (IGF-1). Acylated steryl β-glucoside (PR-ASG) found in pre-germiated brown rice is a bioactive substance exhibiting properties that enhance activity of homocysteine-thiolactonase (HTase), reducing oxidative stress in diabetic neuropathy. The biological importance of PR-ASG in pancreatic β-cells remains unknown. Here we examined the effects of PR-ASG on IGF-1 and glucose metabolism in β-cells exposed to oxidative stress. METHODOLOGY/PRINCIPAL FINDINGS: In the present study, a pre-germinated brown rice (PR)-diet was tested in streptozotocin (STZ)-induced diabetic rats. Compared with diabetic rats fed control diets, the PR-diet fed rats showed an improvement of serum metabolic and neurophysiological parameters. In addition, IGF-1 levels were found to be increased in the serum, liver, and pancreas of diabetic rats fed the PR-diet. The increased IGF-1 level in the pancreas led us to hypothesize that PR-ASG is protective for islet β-cells against the extensive injury of advanced or severe diabetes. Thus we examined PR-ASG to determine whether it showed anti-apoptotic, pro-proliferative effects on the insulin-secreting β-cells line, INS-1; and additionally, whether PR-ASG stimulated IGF-1 autocrine secretion/IGF-1-dependent glucose metabolism. We have demonstrated for the first time that PR-ASG increases IGF-1 production and secretion from pancreatic β-cells. CONCLUSION/SIGNIFICANCE: These findings suggest that PR-ASG may affect pancreatic β-cells through the activation of an IGF-1-dependent mechanism in the diabetic condition. Thus, intake of pre-germinated brown rice may have a beneficial effect in the treatment of diabetes, in particular diabetic neuropathy.
Databáze: OpenAIRE
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