Molecular characterization of Gyps africanus (African white-backed vulture) organic anion transporter 1 and 2 expressed in the kidney
Autor: | Rephima M. Phaswane, Vinny Naidoo, Bono Nethathe, Aron B. Abera |
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Rok vydání: | 2020 |
Předmět: |
Organic anion transporter 1
Physiology NSAIDs Eagles Pharmacology Organic Anion Transporters Sodium-Independent Kidney Toxicology Pathology and Laboratory Medicine Poultry chemistry.chemical_compound 0302 clinical medicine Medicine and Health Sciences Gamefowl 0303 health sciences Analgesics Multidisciplinary biology Eukaryota Drugs Chemistry Toxicity Vertebrates Physical Sciences Medicine Anatomy medicine.drug Research Article Science Excretion Avian Proteins Birds 03 medical and health sciences Diclofenac medicine Animals 030304 developmental biology Raptors Visceral gout Organisms Chemical Compounds Biology and Life Sciences Kidneys Renal System biology.organism_classification Pain management Uric Acid chemistry Gyps africanus Fowl Amniotes biology.protein Uric acid Transcriptome Physiological Processes Gyps Chickens Zoology Acids 030217 neurology & neurosurgery |
Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 5, p e0250408 (2021) |
ISSN: | 1932-6203 |
Popis: | Gyps species have been previously shown to be highly sensitive to the toxic effects of diclofenac, when present in their food sources as drug residues following use as a veterinary medicine. Vultures exposed to diclofenac soon become depressed and die with signs of severe visceral gout and renal damage on necropsy. The molecular mechanism behind toxicity and renal excretion of uric acid is still poorly understood. With the clinical pictures suggesting renal uric acid excretion as the target site for toxicity, as a first step the following study was undertaken to determine the uric acid excretory pathways present in the African white-backed vulture (Gyps africanus) (AWB), one of the species susceptible to toxicity. Using transcriptome analysis, immunohistochemistry and functional predictions, we demonstrated that AWB makes use of the organic anion transporter 2 (OAT2) for their uric acid excretion. RT-qPCR analysis subsequently demonstrated relatively similar expression of the OAT2 transporter in the vulture and chicken. Lastly docking analysis, predicted that the non-steroidal drugs induce their toxicity through an allosteric binding. |
Databáze: | OpenAIRE |
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