Molecular characterization of Gyps africanus (African white-backed vulture) organic anion transporter 1 and 2 expressed in the kidney

Autor: Rephima M. Phaswane, Vinny Naidoo, Bono Nethathe, Aron B. Abera
Rok vydání: 2020
Předmět:
Organic anion transporter 1
Physiology
NSAIDs
Eagles
Pharmacology
Organic Anion Transporters
Sodium-Independent
Kidney
Toxicology
Pathology and Laboratory Medicine
Poultry
chemistry.chemical_compound
0302 clinical medicine
Medicine and Health Sciences
Gamefowl
0303 health sciences
Analgesics
Multidisciplinary
biology
Eukaryota
Drugs
Chemistry
Toxicity
Vertebrates
Physical Sciences
Medicine
Anatomy
medicine.drug
Research Article
Science
Excretion
Avian Proteins
Birds
03 medical and health sciences
Diclofenac
medicine
Animals
030304 developmental biology
Raptors
Visceral gout
Organisms
Chemical Compounds
Biology and Life Sciences
Kidneys
Renal System
biology.organism_classification
Pain management
Uric Acid
chemistry
Gyps africanus
Fowl
Amniotes
biology.protein
Uric acid
Transcriptome
Physiological Processes
Gyps
Chickens
Zoology
Acids
030217 neurology & neurosurgery
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 5, p e0250408 (2021)
ISSN: 1932-6203
Popis: Gyps species have been previously shown to be highly sensitive to the toxic effects of diclofenac, when present in their food sources as drug residues following use as a veterinary medicine. Vultures exposed to diclofenac soon become depressed and die with signs of severe visceral gout and renal damage on necropsy. The molecular mechanism behind toxicity and renal excretion of uric acid is still poorly understood. With the clinical pictures suggesting renal uric acid excretion as the target site for toxicity, as a first step the following study was undertaken to determine the uric acid excretory pathways present in the African white-backed vulture (Gyps africanus) (AWB), one of the species susceptible to toxicity. Using transcriptome analysis, immunohistochemistry and functional predictions, we demonstrated that AWB makes use of the organic anion transporter 2 (OAT2) for their uric acid excretion. RT-qPCR analysis subsequently demonstrated relatively similar expression of the OAT2 transporter in the vulture and chicken. Lastly docking analysis, predicted that the non-steroidal drugs induce their toxicity through an allosteric binding.
Databáze: OpenAIRE
Externí odkaz: