Intraflagellar transport motors in Caenorhabditis elegans neurons
| Autor: | Joshua J. Snow, Amy L. Gunnarson, Jonathan M. Scholey, Guangshuo Ou |
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| Rok vydání: | 2004 |
| Předmět: |
Time Factors
Recombinant Fusion Proteins Green Fluorescent Proteins Mutant Models Biological Biochemistry Intraflagellar transport Fluorescence microscope medicine Animals Cilia Caenorhabditis elegans Neurons Genome Models Genetic biology Chemotaxis Cilium Biological Transport Sequence Analysis DNA biology.organism_classification Cell biology medicine.anatomical_structure Microscopy Fluorescence Multiprotein Complexes Mutation Kinesin sense organs Neuron Signal transduction Signal Transduction |
| Zdroj: | Biochemical Society Transactions. 32:682-684 |
| ISSN: | 1470-8752 0300-5127 |
| DOI: | 10.1042/bst0320682 |
| Popis: | IFT (intraflagellar transport) assembles and maintains sensory cilia on the dendritic endings of chemosensory neurons within the nematode Caenorhabditis elegans. During IFT, macromolecular protein complexes called IFT particles (which carry ciliary precursors) are moved from the base of the sensory cilium to its distal tip by anterograde IFT motors (kinesin-II and Osm-3 kinesin) and back to the base by retrograde IFT-dynein [Rosenbaum and Witman (2002) Nat. Rev. Mol. Cell Biol. 3, 813–825; Scholey (2003) Annu. Rev. Cell Dev. Biol. 19, 423–443; and Snell, Pan and Wang (2004) Cell 117, 693–697]. In the present study, we describe the protein machinery of IFT in C. elegans, which we have analysed using time-lapse fluorescence microscopy of green fluorescent protein-fusion proteins in concert with ciliary mutants. |
| Databáze: | OpenAIRE |
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