Development of a Bead-Based Multiplex Assay for the Analysis of the Serological Response against the Six Pathogens HAV, HBV, HCV, CMV, T. gondii, and H. pylori

Autor: Angela Filomena, Darragh Duffy, Manas K. Akmatov, Gérard Krause, Thomas O. Joos, Barbara Gärtner, Frank Pessler, Matthew L. Albert, Nicole Schneiderhan-Marra, Markus Gerhard
Přispěvatelé: Natural and Medical Sciences Institute [Reutlingen] (NMI), Centre for Experimental and Clinical Infection Research [Hanover] (TWINCORE), Helmholtz Centre for Infection Research (HZI), Centre for Individualized Infection Medicine [Hanover], Translational Infrastructure Epidemiology [Hanover], German Centre for Infection Research (DZIF), Immunobiologie des Cellules dendritiques, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche Translationnelle - Center for Translational Science (CRT), Institut Pasteur [Paris], Institut für Mikrobiologie und Hygiene [Homburg/Saar], Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Part of this work was supported by the European Commission FP7 project SPHINX (grant reference no. 261365). We thank Hannelore Meyer and Luca Formichella, Technical University of Munich, for sending the samples for the H. pylori assay., European Project: 261365,EC:FP7:HEALTH,FP7-HEALTH-2010-single-stage,SPHINX(2010), Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr.7, 38124 Braunschweig, Germany., Vougny, Marie-Christine, Spontaneous clearance in Patients acutely infected with HCV - Immune profiling, Novel biomarkers and X-omics approaches - SPHINX - - EC:FP7:HEALTH2010-11-01 - 2014-04-30 - 261365 - VALID, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7, 38124 Braunschweig, Germany.
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: High-Throughput
High-Throughput, MDPI, 2017, 6 (4), pp.14. ⟨10.3390/ht6040014⟩
High-Throughput; Volume 6; Issue 4; Pages: 14
High-Throughput, Vol 6, Iss 4, p 14 (2017)
High-Throughput, 2017, 6 (4), pp.14. ⟨10.3390/ht6040014⟩
High-throughput
ISSN: 2571-5135
DOI: 10.3390/ht6040014⟩
Popis: International audience; The spread of infectious diseases and vaccination history are common subjects of epidemiological and immunological research studies. Multiplexed serological assays are useful tools for assessing both current and previous infections as well as vaccination efficacy. We developed a serological multi-pathogen assay for hepatitis A, B and C virus, cytomegalovirus (CMV), Toxoplasma gondii, and Helicobacter pylori using a bead-based multiplex assay format. The multi-pathogen assay consisting of 15 antigens was utilized for the analysis of the serological response in elderly individuals of an influenza vaccination study (n = 34). The technical assay validation revealed a mean intra-assay precision of coefficient of variation (CV) = 3.2 ± 1.5% and a mean inter-assay precision of CV = 8.2 ± 5.3% across all 15 antigens and all tested samples, indicating a robust test system. Furthermore, the assay shows high sensitivities (ranging between 94% and 100%) and specificities (ranging between 93% and 100%) for the different pathogens. The highest seroprevalence rates in our cohort were observed for hepatitis A virus (HAV; 73.5%), followed by CMV (70.6%), T. gondii (67.6%) and H. pylori (32.4%). Seroprevalences for hepatitis B virus (HBV, 8.8%) and hepatitis C virus (HCV, 0%) were low. The seroprevalences observed in our study were similar to those from other population-based studies in Germany. In summary, we conclude that our multiplex serological assay represents a suitable tool for epidemiological studies.
Databáze: OpenAIRE
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