An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction
| Autor: | Lijuan Zhang, Kai Lou, Xuee Chen, Xiuqing Gong, Qi Song, Yibo Gao, Rui Tong, Weijia Wen, Xin Tang, Chen Yongsheng, Chuandeng Hu |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Chromatography Materials science sample preparation lcsh:Mechanical engineering and machinery Mechanical Engineering Controller (computing) Extraction (chemistry) Sample processing Pipette DNA 02 engineering and technology 021001 nanoscience & nanotechnology Article nucleic acid 03 medical and health sciences Control and Systems Engineering Nucleic acid RNA lcsh:TJ1-1570 Sample preparation Power output Electrical and Electronic Engineering 0210 nano-technology 030304 developmental biology |
| Zdroj: | Micromachines Volume 10 Issue 3 Micromachines, Vol 10, Iss 3, p 204 (2019) |
| ISSN: | 2072-666X |
| DOI: | 10.3390/mi10030204 |
| Popis: | The result of molecular diagnostic and detection greatly dependent on the quality and integrity of the isolated nucleic acid. In this work, we developed an automated miniaturized nucleic acid extraction device based on magnetic beads method, consisting of four components including a sample processing disc and its associated rotary power output mechanism, a pipetting module, a magnet module and an external central controller to enable a customizable and automated robust nucleic acid sample preparation. The extracted nucleic acid using 293T cells were verified using real-time polymerase chain reaction (PCR) and the data implies a comparable efficiency to a manual process, with the advantages of performing a flexible, time-saving (~10 min), and simple nucleic acid sample preparation. |
| Databáze: | OpenAIRE |
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