Mega-plasmid found worldwide confers multiple antimicrobial resistance in Salmonella Infantis of broiler origin in Russia
| Autor: | Ekaterina V. Krylova, Ekaterina E. Davydova, Alexander A. Komarov, A. N. Bogomazova, Irina V. Soltynskaya, Veronika D. Gordeeva, Olga E. Ivanova |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Salmonella
Virulence Bacterial genome size Biology medicine.disease_cause Microbiology Yersiniabactin Poultry Russia 03 medical and health sciences chemistry.chemical_compound Plasmid Antibiotic resistance Phenols Drug Resistance Multiple Bacterial medicine Animals Humans Israel 030304 developmental biology Genetics Salmonella Infections Animal 0303 health sciences 030306 microbiology Salmonella enterica General Medicine biology.organism_classification Anti-Bacterial Agents Europe Multiple drug resistance Thiazoles chemistry Chickens Genome Bacterial Plasmids Food Science |
| Zdroj: | International Journal of Food Microbiology. 319:108497 |
| ISSN: | 0168-1605 |
| Popis: | Plasmids which are the mobile part of the bacterial genome can acquire and carry over genes conferring antimicrobial resistance, thus contributing to rapid adaptation of bacterial community to human-defined environment. In 2014, Israeli scientists have reported a large conjugative mega-plasmid pESI ( p lasmid for e merging S. Infantis ) that provides multiple drug resistance (MDR) of Salmonella Infantis isolated from broilers. Later, very similar pESI-like plasmids have been found in Salmonella isolated from poultry in the United States, Italy, Switzerland, Hungary, and Japan. Here we report detection of pESI-like plasmids in Salmonella Infantis isolated from chicken food products in Russia. Whole genome sequencing of three MDR isolates revealed pESI-like plasmids in all three cases. These plasmids have such typical pESI features as a locus for siderophore yersiniabactin, a cluster of IncI1 conjugative genes, a cluster of type IV pilus genes, and three toxin-antitoxin modules. The pESI-like plasmids carry from two to five resistance genes in each isolate. In total, we observed six antimicrobial resistance genes associated with pESI-like plasmids (aadA1, blaCTX-M-14, dfrA14, sul1, tetA/tetR, tetM). Besides plasmid genes of antimicrobial resistance, all three MDR isolates of S. Infantis harbor a mutation in chromosomal gene gyrA (p.S83Y or p.D87Y) that is associated with resistance to fluoroquinolones. In addition, we performed a comparative bioinformatics meta-analysis of 25 pESI-like plasmids hosted by S. Infantis from the USA, Europe, Latin America, Israel, and Japan. This analysis identified a 173 kB sequence that is common for all pESI-like plasmids and carries virulence operons and toxin-antitoxin modules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect