Expression and significance of RKIP and E-cadherin in lung squamous cell carcinoma
| Autor: | Dapeng Li, Jing Xie, Xiumin Zhou, Qing-cai Wang, Feng Xiong, Lu Zhang, Jinfang Shi, Chun-rong Zhu |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Cancer Research medicine.medical_specialty Pathology Lung Neoplasms Blotting Western Phosphatidylethanolamine Binding Protein Real-Time Polymerase Chain Reaction Pathology and Forensic Medicine Metastasis Western blot medicine Carcinoma Humans Aged Aged 80 and over Messenger RNA Chi-Square Distribution Clinical pathology medicine.diagnostic_test business.industry Cadherin General Medicine Middle Aged medicine.disease Cadherins Blot Real-time polymerase chain reaction Oncology Carcinoma Squamous Cell Female business |
| Zdroj: | Pathology oncology research : POR. 19(1) |
| ISSN: | 1532-2807 |
| Popis: | The purpose of this study was to investigate the expression of Raf kinase inhibitor protein (RKIP) and epithelial cadherin (E-cadherin) in lung squamous cell carcinoma tissue and its correlation with the clinical pathology of lung squamous cell carcinoma. RKIP and E-cadherin mRNA (by RT-PCR) and protein (by western blotting) levels were monitored in carcinoma tissues and surrounding normal tissues from 86 lung squamous cell carcinoma cases, and their positive rates were calculated. The rates of positive RKIP and E-cadherin mRNA expression were significantly lower in lung squamous cell carcinoma than in the surrounding normal tissues (P 0.05). The positive expression rates were significantly lower in those with lymph node metastasis than in those without (P 0.05). The lower the degree of tumor differentiation, the lower the E-cadherin mRNA positive expression rate (P 0.05). The rates of positive RKIP and E-cadherin mRNA expression were significantly lower in patients at advanced (III, IV) stages than in patients at early (I, II) stages (p 0.05); this rate, however, was independent of gender, age, and tumor size (P 0.05). The protein levels of RKIP and E-cadherin were significantly lower in lung squamous cell carcinoma than in the surrounding normal tissues (P 0.05). The levels were significantly lower in patients with lymph node metastasis than in those without it (P 0.05). The lower the degree of tumor differentiation, the lower the protein level of E-cadherin (P 0.05). Both RKIP and E-cadherin are tumor suppressors, their low expression levels may be associated with initiation, invasion and/or metastasis, as well as with the inhibition of lung squamous cell carcinoma differentiation. |
| Databáze: | OpenAIRE |
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