vB_EcoS_NBD2 bacteriophage-originated polytubes as a carrier for the presentation of foreign sequences
| Autor: | Aurelija Žvirblienė, Aušra Sasnauskienė, Rasa Petraitytė-Burneikienė, Indrė Dalgėdienė, Rasa Insodaitė, Aliona Špakova |
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| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
Recombinant Fusion Proteins Saccharomyces cerevisiae Green Fluorescent Proteins Biology Virus Green fluorescent protein law.invention Bacteriophage 03 medical and health sciences Antigen law Virology Nanotechnology Bacteriophages Histidine Vaccines Virus-Like Particle Antigens 030304 developmental biology chemistry.chemical_classification 0303 health sciences 030306 microbiology biology.organism_classification Fluorescence Amino acid Infectious Diseases chemistry Biochemistry Recombinant DNA Oligopeptides |
| Zdroj: | Virus research. 290 |
| ISSN: | 1872-7492 |
| Popis: | Virus-based nanoparticles constitute a promising platform for the creation of efficient vaccines and nanomaterials. Previously we demonstrated, that the recombinant tail tube protein gp39 of vB_EcoS_NBD2 bacteriophage self-assembles into extremely long (from 0.1 to >3.95 μm), flexible, and stable polytubes when produced in Saccharomyces cerevisiae. To develop a tubular platform for multivalent display of foreign antigens, yeast-derived recombinant tail tube protein gp39 was chosen as a scaffold. The carboxy-terminal fusions of gp39 with various antigens up to 238 amino acids in length resulted in different synthesis efficiency and self-assembly capacity. Recombinant gp39 fused with green fluorescent protein (eGFP) comprising 238 amino acid residues was capable to self-assemble into short fluorescent polytubes with retained eGFP functional activity. By demonstrating the display of active foreign antigens on the exterior surface of polytubes, these structures may provide a promising tool for diverse applications in nanotechnology. |
| Databáze: | OpenAIRE |
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