Electrospun PCL nanofibers blended with Wattakaka volubilis active phytochemicals for bone and cartilage tissue engineering
| Autor: | Selvakumar Rajendran, Amitava Bhattacharyya, K. Santosh Sahanand, Elakkiya Venugopal |
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| Rok vydání: | 2019 |
| Předmět: |
Scaffold
Bone Regeneration Polyesters Phytochemicals Nanofibers Palmitic Acid Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Biodegradable Plastics 02 engineering and technology Cell Line 03 medical and health sciences chemistry.chemical_compound medicine Humans Meniscus General Materials Science Fourier transform infrared spectroscopy 030304 developmental biology 0303 health sciences Osteoblasts Tissue Engineering Chemistry Osteoblast 021001 nanoscience & nanotechnology Electrospinning Apocynaceae Hoechst stain Cartilage medicine.anatomical_structure Chemical engineering Phytochemical Nanofiber Polycaprolactone Microscopy Electron Scanning Molecular Medicine 0210 nano-technology Stearic Acids |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 21:102044 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2019.102044 |
| Popis: | In the present study, the polycaprolactone (PCL) nanofibers were investigated as a carrier to deliver phytochemicals for bone and cartilage tissue engineering. The PCL nanofibers was blended with phytochemicals hexadecanoic acid, octadecanoic acid and N,N-diisopropyl (2,2,3,3,3-pentafluoropropyl) amine isolated from a medicinal plant, Wattakaka volubilis. The scaffolds were characterized using scanning electron microscope (SEM) and Fourier transform infrared (FTIR) spectroscopy. The average diameter of control and phytochemical loaded nanofiber was 208 ± 9.6 nm and 316 ± 7.0 nm respectively. Biodegradation rate of nanofibers, impact of nanofiber on meniscus and osteoblast cell growth was analyzed using 3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) assay, DNA content and extra cellular matrix secretion. Hoechst stain and SEM images were used to visualize and monitor the cell growth on PCL scaffold. The phytochemicals incorporated PCL nanofibers enhanced the growth and proliferation of primary human meniscus and osteoblast like cells and hence may be suitable scaffold for bone and cartilage tissue engineering applications. |
| Databáze: | OpenAIRE |
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