Characterization of Canadian cefoxitin-resistant non-typhoidal Salmonella isolates, 2005-06
| Autor: | Laura F. Mataseje, K. Doré, J. Xiao, Sara E.F. Kost, Michael R. Mulvey, L.-K. Ng |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
DNA Bacterial Male Microbiology (medical) Canada Salmonella Genotype Microbial Sensitivity Tests Biology medicine.disease_cause Polymerase Chain Reaction Microbiology Cefoxitin Young Adult Plasmid Drug Resistance Bacterial medicine Humans Pharmacology (medical) Replicon Aged Antibacterial agent Aged 80 and over Pharmacology Sequence Analysis DNA Middle Aged biochemical phenomena metabolism and nutrition bacterial infections and mycoses Virology Anti-Bacterial Agents Multiple drug resistance Infectious Diseases Genes Bacterial Salmonella Infections Female Restriction fragment length polymorphism Polymorphism Restriction Fragment Length Plasmids medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 64:723-730 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkp249 |
| Popis: | OBJECTIVES: Resistance to extended-spectrum cephalosporins has increased in Salmonella worldwide, and is a concern in both hospital and community settings. The aim of this report was to investigate cefoxitin-resistant Salmonella isolates identified from human clinical cases across Canada. METHODS: Cefoxitin-resistant isolates, defined as having an MIC > or = 32 mg/L, were screened for the ampC classes DHA, FOX, ENT and CIT in a multiplex PCR followed by sequence analysis. Plasmid analysis by restriction fragment length polymorphism (RFLP) and replicon typing was performed on a convenience sample of cefoxitin-resistant Salmonella. RESULTS: In 2005, 5.3% (181/3442) and in 2006, 3.1% (102/3250) of Salmonella isolates collected from all provinces across Canada displayed cefoxitin resistance. Seventy-one out of 283 (25.1%) were multidrug resistant (MDR), as defined by resistance to at least three different antibiotic classes. The bla(CMY-2) gene was harboured by 96.8% (274/283) of the cefoxitin-resistant isolates. Analysis of CMY-2 plasmids revealed that 19.7% contained genes conferring resistance to multiple antimicrobials. Replicon typing of transformant CMY-2 plasmid DNA revealed the predominance of I1-Igamma and A/C. Of the MDR CMY-2 plasmids, 75% contained replicon type A/C. RFLP patterns of CMY-2 plasmids revealed clusters corresponding to the I1-Igamma and A/C replicon types. CONCLUSIONS: Incompatibility group I1-Igamma is the most prevalent of the Salmonella CMY-2 plasmids, while A/C is associated with MDR CMY-2 plasmids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|