The Arabidopsis acetylated histone-binding protein BRAT1 forms a complex with BRP1 and prevents transcriptional silencing
| Autor: | Huan-Wei Huang, Cui-Jun Zhang, Lin Li, Lian-Mei Tan, Xin-Jian He, Tao Cai, Yong-Qiang Li, Chang-Rong Shao, She Chen, Xiao-Mei Hou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Science Arabidopsis General Physics and Astronomy Biology Models Biological Article General Biochemistry Genetics and Molecular Biology Histones 03 medical and health sciences Protein Domains Transcription (biology) Gene Silencing RNA Messenger Genetics Multidisciplinary Arabidopsis Proteins Acetylation General Chemistry DNA Methylation Argonaute Bromodomain DNA Demethylation 030104 developmental biology Histone DNA demethylation Genetic Loci RNA Plant DNA glycosylase Mutation DNA methylation biology.protein Protein Binding |
| Zdroj: | Nature Communications, Vol 7, Iss 1, Pp 1-13 (2016) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Transposable elements and other repetitive DNA sequences are usually subject to DNA methylation and transcriptional silencing. However, anti-silencing mechanisms that promote transcription in these regions are not well understood. Here, we describe an anti-silencing factor, Bromodomain and ATPase domain-containing protein 1 (BRAT1), which we identified by a genetic screen in Arabidopsis thaliana. BRAT1 interacts with an ATPase domain-containing protein, BRP1 (BRAT1 Partner 1), and both prevent transcriptional silencing at methylated genomic regions. Although BRAT1 mediates DNA demethylation at a small set of loci targeted by the 5-methylcytosine DNA glycosylase ROS1, the involvement of BRAT1 in anti-silencing is largely independent of DNA demethylation. We also demonstrate that the bromodomain of BRAT1 binds to acetylated histone, which may facilitate the prevention of transcriptional silencing. Thus, BRAT1 represents a potential link between histone acetylation and transcriptional anti-silencing at methylated genomic regions, which may be conserved in eukaryotes. Transposons and repetitive sequences are typically subject to transcription silencing. Here, Zhang et al. find that the bromodomain-containing protein BRAT1 forms a complex with BRP1, recognizes histone acetylation and acts to prevent transcriptional silencing in Arabidopsis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|