The effect of nacre extract on cord blood‐derived endothelial progenitor cells: A natural stimulus to promote angiogenesis?

Autor: Emilie Velot, Pierre Gillet, Véronique Decot, Ganggang Zhang, Marthe Rousseau, Arnaud Bianchi, Vanessa Moby, Anne-Sophie Willemin
Přispěvatelé: Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), The First Affiliated Hospital of Zhengzhou University, Faculté de Pharmacie [Nancy], Université de Lorraine (UL), unité de thérapie cellulaire et Tissus (UTCT), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), INSERM U1059, SAINBIOSE - Santé, Ingénierie, Biologie, Saint-Etienne (SAINBIOSE-ENSMSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'Odontologie [CHRU Nancy], Rousseau, Marthe, Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté d'odontologie [Nancy]
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Programmed cell death
Materials science
Angiogenesis
[SDV]Life Sciences [q-bio]
0206 medical engineering
Cell
Becaplermin
Biomedical Engineering
Neovascularization
Physiologic
02 engineering and technology
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
stimulus
Biomaterials
angiogenesis
medicine
[SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Animals
Progenitor cell
ComputingMilieux_MISCELLANEOUS
endothelial progenitor cells
Tube formation
ethanol soluble matrix
Metals and Alloys
Fetal Blood
021001 nanoscience & nanotechnology
020601 biomedical engineering
In vitro
Extracellular Matrix
Cell biology
Endothelial stem cell
medicine.anatomical_structure
Gene Expression Regulation
Matrix Metalloproteinase 9
nacre
Cord blood
Ceramics and Composites
Matrix Metalloproteinase 2
0210 nano-technology
Zdroj: Journal of Biomedical Materials Research Part A
Journal of Biomedical Materials Research Part A, 2019, 107 (7), pp.1406-1413. ⟨10.1002/jbm.a.36655⟩
Journal of Biomedical Materials Research Part A, Wiley, 2019, 107 (7), pp.1406-1413. ⟨10.1002/jbm.a.36655⟩
ISSN: 1549-3296
1552-4965
Popis: Angiogenesis is a critical parameter to consider for the development of tissue-engineered bone substitutes. The challenge is to promote sufficient vascularization in the bone substitute to prevent cell death and to allow its efficient integration. The capacity of nacre extract to restore the osteogenic activity of osteoarthritis osteoblasts has already been demonstrated. However, their angiogenic potential on endothelial progenitor cells (EPCs) was not yet explored. Therefore, the current study aimed at investigating if nacreous molecules affect EPC behavior. The gene and protein expression levels of endothelial cell (EC)-specific markers were determined in EPCs cultivated in presence of a nacre extract (ethanol soluble matrix [ESM] at two concentrations: 100 μg/mL and 200 μg/mL (respectively abbreviated ESM100 and ESM200)). Cell functionality was explored by proangiogenic factors production and in vitro tube formation assay. ESM200 increased the expression of some EC-specific genes. The in vitro tube formation assay demonstrated that ESM200 stimulated tubulogenesis affecting angiogenic parameters. We demonstrated that a stimulation with 200 μg/mL of ESM increased angiogenesis key elements. This in vitro study strongly highlights the proangiogenic effect of ESM. Due to its osteogenic properties, previously demonstrated, ESM could constitute the key element to develop an ideal prevascularized bone substitute. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.
Databáze: OpenAIRE
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