Detection of prostate-specific membrane antigen on HUVECs in response to breast tumor-conditioned medium
| Autor: | Tiancheng Liu, Mohamed Jabbes, Clifford E. Berkman, Lisa Y. Wu, Jessie R. Nedrow-Byers, Jeffrey N. Bryan |
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| Rok vydání: | 2010 |
| Předmět: |
Glutamate Carboxypeptidase II
Vascular Endothelial Growth Factor A Cancer Research Pathology medicine.medical_specialty Umbilical Veins Angiogenesis Breast Neoplasms Biology urologic and male genital diseases Umbilical vein chemistry.chemical_compound Cell Line Tumor medicine Humans Cells Cultured Tube formation Matrigel Neovascularization Pathologic Endothelial Cells Endothelial stem cell Vascular endothelial growth factor Vascular endothelial growth factor A Oncology chemistry Culture Media Conditioned Cancer cell Antigens Surface Cancer research Female |
| Zdroj: | International journal of oncology. 38(5) |
| ISSN: | 1791-2423 |
| Popis: | Prostate-specific membrane antigen (PSMA), a well-known biomarker of prostate cancer, has also been found to be highly expressed in the neovasculature of multiple non-prostatic solid tumors. As a consequence, it has the potential to become a biomarker for tumor-associated vasculature. Herein, we describe an in vitro model for assessing PSMA expression associated with tube formation by primary human umbilical vein endothelial cells (HUVECs) cultured in Matrigel and induced by tumor-conditioned medium (TCM) derived from human breast cancer cells (MDA-MB-231). In contrast to vascular endothelial growth factor (VEGF)-containing endothelial cell medium, TCM induced higher expression of PSMA in HUVECs. The vessel-like tubes were detected by imaging with fluorescent PSMA inhibitors. Consequently, this in vitro model is expected to enable subsequent studies aimed at determining the role of PSMA in angiogenesis and factors that induce it. |
| Databáze: | OpenAIRE |
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