Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of Heliothis virescens ascovirus 3 h (HvAV-3h)
| Autor: | Ning-Ning Han, Guo-Hua Huang, Hu Wan, Zi-Shu Chen, Jianhong Li |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Science Biology Real-Time Polymerase Chain Reaction 01 natural sciences Ribosome Article 03 medical and health sciences chemistry.chemical_compound Ribosomal protein Ascoviridae Reference genes Animals Gene Genetics Multidisciplinary Heliothis virescens Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Reference Standards biology.organism_classification Hymenoptera Eukaryotic translation elongation factor 1 alpha 1 Lepidoptera Elongation factor 010602 entomology 030104 developmental biology chemistry Medicine DNA |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-07684-w |
| Popis: | Ascoviruses are double-stranded DNA viruses that mainly infect noctuid larvae, and are transmitted by the parasitoid wasp Microplitis similis Lyle. Ascovirus-parasitoids wasp-noctuid insects constitute the dissemination system. Selection of suitable reference genes for the dissemination system could play an important role in elucidating the pathogenic molecular mechanisms of ascovirus. Unfortunately, such studies on potential reference genes in the dissemination system of ascoviruses are lacking. In the present study, we evaluated 11 candidate reference genes: β-actin1 (ACT1), β-actin2 (ACT2), elongation factor 1 (EF1), elongation factor 2 (EF2), ribosomal protein L10 (L10), ribosomal protein L17A (L17A), superoxide dismutase (SOD), 28S ribosome (28S), Tubulin (TUB) and 18S ribosome (18S). The samples were originally from various virus concentrations and points-in-time of experimental treatments using RefFinder and four algorithms. The results showed that EF1 was the most stable internal gene in S. exigua and M. similis and that EF2 was the most stable in the IOZCAS-Spex-II-A cell line, and the stability of reference genes were confirmed via the expression levels of two inhibitor of apoptosis-like (iap-like) genes from Heliothis virescens ascovirus 3 h (HvAV-3h). This study provides a crucial basis for future research that explores the molecular mechanisms of the pathogenesis of ascoviruses. |
| Databáze: | OpenAIRE |
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