Lebectin increases N-cadherin-mediated adhesion through PI3K/AKT pathway

Autor: Mohamed El Ayeb, Naziha Marrakchi, Carole Siret, Frédéric André, Sameh Sarray, José Luis, Maxime Lehmann
Přispěvatelé: Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), Faculté des Sciences, Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis (FST), Université de Tunis El Manar (UTM)-Université de Tunis El Manar (UTM), Centre de Recherches en Oncologie biologique et Oncopharmacologie (CRO2), Aix Marseille Université (AMU)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté de Médecine de Tunis, Université de Tunis El Manar (UTM)
Rok vydání: 2009
Předmět:
MESH: Signal Transduction
Cancer Research
MESH: Cell Line
Tumor
Blotting
Western
Integrin
Viper Venoms
Biology
MESH: Cadherins
MESH: Cell Adhesion
Phosphatidylinositol 3-Kinases
03 medical and health sciences
0302 clinical medicine
Cell–cell interaction
Cell Movement
Cell Line
Tumor
Cell Adhesion
Humans
Immunoprecipitation
MESH: Blotting
Western
Lectins
C-Type
CD93
Cell adhesion
MESH: Cell Movement
030304 developmental biology
0303 health sciences
MESH: Humans
MESH: Proto-Oncogene Proteins c-akt
MESH: Immunoprecipitation
Cell adhesion molecule
Cadherin
MESH: Immunohistochemistry
Cadherins
Intercellular adhesion molecule
Immunohistochemistry
3. Good health
Cell biology
Oncology
MESH: Phosphatidylinositol 3-Kinases
030220 oncology & carcinogenesis
biology.protein
[SDV.IMM]Life Sciences [q-bio]/Immunology
Neural cell adhesion molecule
MESH: Viper Venoms
Proto-Oncogene Proteins c-akt
MESH: Lectins
C-Type
Signal Transduction
Zdroj: Cancer Letters
Cancer Letters, Elsevier, 2009, 285 (2), pp.174-81. ⟨10.1016/j.canlet.2009.05.012⟩
ISSN: 0304-3835
Popis: International audience; Cell adhesion molecules, including cadherins and integrins, play an essential role during tumor progression and represent potential targets for the development of new therapeutic agents. We previously showed that lebectin, a C-type lectin protein (CLP) issued from Macrovipera lebectina snake venom, inhibits integrin-mediated migration of IGR39 melanoma cells. Here we assessed whether lebectin modulates cell-cell adhesion. We demonstrated that lebectin promotes N-cadherin/catenin complex reorganization at cell-cell contacts, inducing a strengthening of intercellular adhesion. This reorganization is associated to phosphorylation of beta-catenin on tyrosine 142 residue. Interestingly, lebectin acts on N-cadherin-mediated cell-cell contacts through PI3K/Akt pathway. This effect could contribute to the blockage of tumor cell migration previously observed.
Databáze: OpenAIRE
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