Translocation arrest of an intramitochondrial sorting signal next to Tim11 at the inner-membrane import site
| Autor: | K, Tokatlidis, T, Junne, S, Moes, G, Schatz, B S, Glick, N, Kronidou |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Mitochondrial intermembrane space Translocase of the outer membrane Recombinant Fusion Proteins Molecular Sequence Data Dithionitrobenzoic Acid Saccharomyces cerevisiae Biology Protein Sorting Signals medicine.disease_cause Membrane Potentials Fungal Proteins Adenosine Triphosphate Protein targeting medicine Inner membrane Amino Acid Sequence Protein Precursors Inner mitochondrial membrane Multidisciplinary L-Lactate Dehydrogenase Membrane Proteins Biological Transport Intracellular Membranes Mitochondrial Proton-Translocating ATPases Cell biology Mitochondria Tetrahydrofolate Dehydrogenase Cross-Linking Reagents Biochemistry Translocase of the inner membrane L-Lactate Dehydrogenase (Cytochrome) Intermembrane space Bacterial outer membrane Carrier Proteins |
| Zdroj: | Nature. 384(6609) |
| ISSN: | 0028-0836 |
| Popis: | THE import of proteins from the cytosol into the mitochondrial matrix involves the concerted action of two separate import systems: the TOM system in the outer membrane, and the TIM system in the inner membrane1,2. Here we report that the inner-membrane system also sorts proteins to the intermembrane space. Some intermembrane-space proteins, such as cytochromes b2 and c1, are synthesized with a complex pre-sequence consisting of a positively charged matrix targeting signal followed by an uncharged sequence that acts as sorting signal for the intermem-brane space3. We show that this sorting signal can be efficiently crosslinked to an inner-membrane protein of relative molecular mass 11K after the mature part of the precursor has been sorted to the intermembrane space. The UK protein, which we term Tim11, is a component of the protein import system in the inner membrane. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|