Long chain polyunsaturated fatty acids (LCPUFAs) and nordihydroguaiaretic acid (NDGA) modulate metabolic and inflammatory markers in a spontaneous type 2 diabetes mellitus model (Stillman Salgado rats)
| Autor: | Aldo R. Eynard, Alejandro Dain, Jairam Vanamala, Gustavo Tomás Díaz-Gerevini, Undurti N. Das, Gastón Repossi |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Plasma Triglycerides Endocrinology Diabetes and Metabolism Clinical Biochemistry Type 2 diabetes chemistry.chemical_compound 0302 clinical medicine Nordihydroguaiaretic acid Endocrinology Hyperinsulinemia Gamma-glutamyltransferase chemistry.chemical_classification eSS rats (Stillman Salgado rats) Arachidonic Acid biology Nordihydroguaiaretic Acid Otras Medicina Básica gamma-Glutamyltransferase Chronic inflammation purl.org/becyt/ford/3.1 [https] Type 2 Diabetes Medicina Básica C-Reactive Protein Arachidonic acid purl.org/becyt/ford/3 [https] Lipidology Polyunsaturated fatty acid medicine.medical_specialty Plasma triglycerides CIENCIAS MÉDICAS Y DE LA SALUD Ess Rats (Stillman Salgado Rats) 030209 endocrinology & metabolism 03 medical and health sciences Internal medicine Diabetes mellitus Fatty Acids Omega-3 Chronic Inflammation medicine Animals Masoprocol Rats Wistar Triglycerides Glycated Hemoglobin Inflammation Biochemistry medical Interleukin-6 Research Biochemistry (medical) Hypertriglyceridemia Pufas medicine.disease Rats Oxidation Process Disease Models Animal 030104 developmental biology chemistry Diabetes Mellitus Type 2 Dietary Supplements biology.protein PUFAs Oxidation process Biomarkers |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Lipids in Health and Disease |
| DOI: | 10.1186/s12944-016-0363-8 |
| Popis: | Diabetes mellitus (DM) is a complex disease with alterations in metabolic and inflammatory markers. Stillman Salgado rats (eSS) spontaneously develop type 2 DM by middle age showing progressive impairment of glucose tolerance with hyperglycemia, hypertriglyceridemia and hyperinsulinemia. We analyzed the effects of supplementation of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs) with or without nordihydroguaiaretic acid (NDGA) added, an antioxidant and lipoxygenase inhibitor, on metabolic and inflammatory parameters in eSS rats to evaluate whether they can delay development and/or prevent progression of DM. Methods: After weaning, eSS rats received, intraperitoneally, once a month ω-3 (EPA 35% and DHA 40%-6.25 mg/Kg) or ω-6 (90% arachidonic acid- 6. 25 mg/Kg) for twelve months. Two additional groups of rats received 1.9 mg/kg NDGA added to ω-3 and ω-6 fatty acids. Blood samples were collected at day 40, and at the end of the 6th month and 12th month of age to determine plasma triglycerides (TGs), total plasma fatty acids (FA), A1C hemoglobin (HbA1C), C-reactive protein (CRP), gamma glutamyl transpeptidase (GGT), lipo and hydro peroxides, nitrites and IL-6 (in plasma and liver, kidney, and pancreas) and underwent oral glucose tolerance test (OGTT) as well. Wistar and eSS rats that received saline solution were used as controls. Results: Plasma lipids profile, TG, fasting and post-prandial blood glucose levels, and glycosylated HbA1C showed significant improvements in ω-3 and ω-3 + NDGA treated animals compared to eSS control group. ω-3 and ω-3 + NDGA groups showed an inverse correlation with fasting blood glucose and showed lower plasma levels of GGT, TG, and CRP. eSS rats treated with ω-3 LCPUFAs showed reduced level of inflammatory and oxidative indices in plasma and liver, kidney and pancreas tissues in comparison with eSS control (non-treated) and ω-6 treated groups. Conclusions: eSS rats are a useful model to study type 2 DM pathophysiology and related inflammatory indices. ω-3 + NDGA supplementation, at the doses tested, ameliorated inflammatory, metabolic and oxidative stress markers studied. Fil: Dain, Alejandro. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Histología,embriología y Genética I; Argentina Fil: Repossi Marquez, Pablo Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: Diaz Gerevini, Gustavo T.. Universidad Nacional de Córdoba. Facultad de Medicina. Cátedra de Histología,embriología y Genética I; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: Vanamala, Jairam. State University of Pennsylvania; Estados Unidos Fil: Das, Undurti N.. Gayatri Vidya Parishad Institue of Health Care and Medical Technology; India Fil: Eynard, Aldo Renato. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina |
| Databáze: | OpenAIRE |
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