Complete genome sequence of cherry virus T, a novel cherry-infecting tepovirus
Autor: | D. Cornaggia, Chantal Faure, Y. Brans, Thierry Candresse, J. Suchá, Milan Navrátil, Dana Šafářová, Armelle Marais |
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Přispěvatelé: | Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Palacky University Olomouc, Ctifl - Centre de Lanxade (Ctifl - Centre de Lanxade ), Centre Technique Interprofessionnel des Fruits et Légumes (CTIFL), Research and Breeding Institute of Pomology Holovousy (VSUO) |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
viruses
[SDV]Life Sciences [q-bio] Virologie végétale Séquence génomique Prune dwarf virus Pathologie végétale Genome Viral Virus phytopathogène Prunus avium Genome Virus 03 medical and health sciences Open Reading Frames Virology Tepovirus [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Arbre fruitier à noyau Movement protein Phylogeny 030304 developmental biology Plant Diseases 0303 health sciences Génome biology Base Sequence Whole Genome Sequencing 030306 microbiology High-Throughput Nucleotide Sequencing General Medicine biology.organism_classification Prunus cerasus [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy Santé des plantes prunus cerasus Novel virus Prunus necrotic ringspot virus prunus virus T Flexiviridae Cerisier |
Zdroj: | Archives of Virology Archives of Virology, Springer Verlag, 2020, 165, pp.1711-1714. ⟨10.1007/s00705-020-04656-w⟩ |
ISSN: | 0304-8608 1432-8798 |
DOI: | 10.1007/s00705-020-04656-w⟩ |
Popis: | International audience; Double-stranded RNA and total RNA purified from sour cherry leaves (Prunus cerasus, cv. Amarelka Chvalkovicka) was analyzed by high-throughput sequencing. BLAST annotation identified contigs with homology to several already known cherry-infecting viruses (prune dwarf virus, prunus necrotic ringspot virus, prunus virus F, little cherry virus 1) as well as contigs with sequences more distantly related to those of members of the family Betaflexiviridae and in particular to prunus virus T of the genus Tepovirus. The full genome sequence of a putative virus (6,847 nucleotides [nt]; GenBank no. MT090966) was assembled and completed at the genome ends. The genome has a typical tepovirus organization, containing three overlapping open reading frames (ORFs), encoding a replication-associated protein, a movement protein and a capsid protein, respectively. Both its genome organization and its phylogenetic relationships show that the virus belongs to the genus Tepovirus, but considering the species demarcation criteria for the family Betaflexiviridae, it appears to represent a novel virus species, and we propose the name "cherry virus T" (ChVT) for this virus. |
Databáze: | OpenAIRE |
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