Dihydropyridine-derived BODIPY probe for detecting exogenous and endogenous nitric oxide in mitochondria
| Autor: | Lan Lin, Chang Gao, Jun-Ru Huang, Lan He, Zhong-Lin Lu, Wan Sun, Zheng-Li Tan |
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| Rok vydání: | 2017 |
| Předmět: |
Boron Compounds
endocrine system Dihydropyridines Cell Survival chemistry.chemical_element 02 engineering and technology 010402 general chemistry Photochemistry Nitric Oxide 01 natural sciences Oxygen Analytical Chemistry Nitric oxide chemistry.chemical_compound Mice Organophosphorus Compounds Pyridine medicine Animals Humans Fluorescent Dyes Aromatization Dihydropyridine Hep G2 Cells 021001 nanoscience & nanotechnology Fluorescence Nitrogen 0104 chemical sciences Mitochondria RAW 264.7 Cells chemistry Biophysics BODIPY 0210 nano-technology medicine.drug |
| Zdroj: | Talanta. 176 |
| ISSN: | 1873-3573 |
| Popis: | A mitochondria-targetable probe Mito-DHP for nitric oxide (NO) was designed and synthesized by introducing dihydropyridine and triphenylphosphonium (TPP) moieties into boron dipyrromethene (BODIPY) dye. Mito-DHP was able to effectively detect nitric oxide through the aromatization of dihydropyridine to fluorescent pyridine product under oxygen-free conditions. The probe Mito-DHP showed high selectivity to NO over a number of reactive oxygen/nitrogen species (ROS/RNS) as well as high sensitivity (detection limit at 25nM), pH stability and bio-compatibility. Furthermore, Mito-DHP proved to target mitochondria specifically and to visualize both exogenous and endogenous NO in real time. |
| Databáze: | OpenAIRE |
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