The macrophage-expressed gene (mpeg) 1 identifies a subpopulation of B cells in the adult zebrafish

Autor: Etienne Gomez, Sowmya lyer, Valérie Wittamer, Mireia Rovira, Julien Y. Bertrand, Magali Miserocchi, David M. Langenau, Giuliano Ferrero
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
mpeg1
ddc:616.07
0302 clinical medicine
Immunology and Allergy
Macrophage
Myeloid Cells
Tissue Distribution
Skin/cytology/metabolism
Transgenes
Zebrafish
Skin
0303 health sciences
education.field_of_study
B-Lymphocytes
biology
Myeloid Cells/metabolism
Perforin‐2
Mononuclear phagocyte system
Membrane Proteins/genetics/metabolism
Sciences bio-médicales et agricoles
Perforin-2
Cell biology
Haematopoiesis
030220 oncology & carcinogenesis
Interferon Regulatory Factors
Myelopoiesis
Single-Cell Analysis
Immunology
Population
Zebrafish Proteins/genetics/metabolism
Cell Development
Differentiation
& Trafficking
Article
Zebrafish/genetics
03 medical and health sciences
Phagocytosis
Macrophages/metabolism
Animals
B-Lymphocytes/cytology/metabolism
education
030304 developmental biology
Macrophages
Membrane Proteins
Cell Biology
Zebrafish Proteins
biology.organism_classification
030104 developmental biology
Gene Expression Regulation
IRF8
mCherry
030215 immunology
Interferon Regulatory Factors/metabolism
B lymphocytes
Zdroj: Journal of leukocyte biology, 107 (3
Journal of Leukocyte Biology, Vol. 107, No 3 (2020) pp. 431-443
Journal of Leukocyte Biology
ISSN: 0741-5400
Popis: The mononuclear phagocytic system consists of many cells, in particular macrophages, scattered throughout the body. However, there is increasing evidence for the heterogeneity of tissue‐resident macrophages, leading to a pressing need for new tools to discriminate mononuclear phagocytic system subsets from other hematopoietic lineages. Macrophage‐expressed gene (Mpeg)1.1 is an evolutionary conserved gene encoding perforin‐2, a pore‐forming protein associated with host defense against pathogens. Zebrafish mpeg1.1:GFP and mpeg1.1:mCherry reporters were originally established to specifically label macrophages. Since then more than 100 peer‐reviewed publications have made use of mpeg1.1‐driven transgenics for in vivo studies, providing new insights into key aspects of macrophage ontogeny, activation, and function. Whereas the macrophage‐specific expression pattern of the mpeg1.1 promoter has been firmly established in the zebrafish embryo, it is currently not known whether this specificity is maintained through adulthood. Here we report direct evidence that beside macrophages, a subpopulation of B‐lymphocytes is marked by mpeg1.1 reporters in most adult zebrafish organs. These mpeg1.1 + lymphoid cells endogenously express mpeg1.1 and can be separated from mpeg1.1 + macrophages by virtue of their light‐scatter characteristics using FACS. Remarkably, our analyses also revealed that B‐lymphocytes, rather than mononuclear phagocytes, constitute the main mpeg1.1‐positive population in irf8 null myeloid‐defective mutants, which were previously reported to recover tissue‐resident macrophages in adulthood. One notable exception is skin macrophages, whose development and maintenance appear to be independent from irf8, similar to mammals. Collectively, our findings demonstrate that irf8 functions in myelopoiesis are evolutionary conserved and highlight the need for alternative macrophage‐specific markers to study the mononuclear phagocytic system in adult zebrafish.
Mpeg1 not only is a restricted macrophage marker, but also labels B cells in the adult zebrafish; thus previously identified irf8‐independent macrophages likely consist of B lymphocytes.
Databáze: OpenAIRE
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