3D-printed rolling circle amplification chip for on-site colorimetric detection of inorganic mercury in drinking water
| Autor: | Sung-Wook Choi, Ji Won Lim, Min-Ah Woo, Tai-Yong Kim |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Deoxyribozyme
chemistry.chemical_element 01 natural sciences Sensitivity and Specificity Analytical Chemistry law.invention chemistry.chemical_compound 0404 agricultural biotechnology Magazine law Reproducibility Chromatography Drinking Water 010401 analytical chemistry Reproducibility of Results 04 agricultural and veterinary sciences General Medicine DNA Catalytic Equipment Design Mercury Chip 040401 food science 0104 chemical sciences Thymine Mercury (element) chemistry Rolling circle replication Spectrophotometry Printing Three-Dimensional Colorimetry Selectivity Water Pollutants Chemical Food Science |
| Zdroj: | Food chemistry. 300 |
| ISSN: | 1873-7072 |
| Popis: | A point-of-care testing chip was developed for the colorimetric detection of inorganic mercury ion (Hg2+). The disposable chip fabricated by three-dimensional printing technology contains DNAzymes produced by rolling circle amplification (RCA); a color change caused by the enzymatic reaction between DNAzymes and the peroxidase substrate 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is measured using a portable spectrophotometer. In the “turn-off”-type RCA reaction, the annealing of the T(12) primer that initiates the RCA reaction is blocked by the interaction of thymine with Hg2+; thus, the amount of amplified DNAzymes causing a color change is decreased depending on Hg2+ concentration. The colorimetric signal is enhanced by amplifying double-repeat DNAzymes from a circular DNA template. The chip detected Hg2+ in tap drinking water samples with high sensitivity (lowest validated value: 3.6 μg/L) and showed better selectivity, precision, and reproducibility than conventional analysis instruments. This low-cost easy-to-use platform can reduce the risk of accidental Hg2+ poisoning. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect