Chitosan Acetylation Degree Influences the Physical Properties of Polysaccharide Nanoparticles: Implication for the Innate Immune Cells Response
| Autor: | Pasquale Sacco, Eva Decleva, Eleonora Marsich, Ivan Donati, Sergio Paoletti, Renzo Menegazzi, Franco Furlani |
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| Přispěvatelé: | Furlani, Franco, Sacco, Pasquale, Decleva, Eva, Menegazzi, Renzo, Donati, Ivan, Paoletti, Sergio, Marsich, Eleonora |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Cell Survival Nanoparticle Inflammation 02 engineering and technology macrophage Polysaccharide Cell Line Degree (temperature) Chitosan 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems neutrophils Polysaccharides medicine Humans chitosan acetylation degree inflammation macrophages nanoparticles stability Materials Science (all) General Materials Science Hyaluronic Acid 030304 developmental biology chemistry.chemical_classification 0303 health sciences Innate immune system nanoparticle technology industry and agriculture neutrophil Acetylation 021001 nanoscience & nanotechnology Immunity Innate carbohydrates (lipids) chemistry Biophysics medicine.symptom Reactive Oxygen Species 0210 nano-technology |
| Popis: | The aim of the present contribution is twofold as it reports (i) on the role played by chitosan acetylation degree for the stability of nanoparticles (NPs) formed with hyaluronan and (ii) on the effect of the interaction of such NPs with immune cells. Chitosans with similar viscosity-average molecular weight, Mv, (i.e., 200 000) and different fractions of acetylated units (F A ) together with low-molecular-weight hyaluronan were chosen for developing a select library of formulations via electrostatic complex coacervation. The resulting NPs were analyzed in terms of size, polydispersity, surface charge, and stability in physiological-mimicked media by dynamic light scattering. Only medium acetylated chitosan (F A = 0.16) guaranteed the stability of NPs. To explore the effect of NPs interaction with immune cells, the release of proinflammatory cytokines and the reactive oxygen species production by human macrophages and neutrophils, respectively, were evaluated. Strikingly, a structure-function relationship emerged, showing that NPs made of chitosans with F A = 0.02, 0.25, 0.46, and 0.63 manifested a proinflammatory activity, linked to the instability of the system. Conversely, NPs made of chitosan with F A = 0.16 neither modified the functional response of macrophages nor that of neutrophils. Of note, such NPs were found to possess additional properties potentially advantageous in applications such as delivery of therapeutics to target inflamed sites: (i) they are devoid of cytotoxic effects, (ii) they avoid engulfment during the early stage of interaction with macrophages, and (iii) they are muco-adhesive, thereby providing for site-specificity and long-residence effects |
| Databáze: | OpenAIRE |
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