Bone marrow-derived mesenchymal stem cells modulate BV2 microglia responses to lipopolysaccharide
| Autor: | Shi Wei Tan, Rajesh Ramasamy, Zul ' atfi Rahmat, Hemavathy Subramaiam, Yin Yin Ooi, Sharmili Vidyadaran, Daud Ahmad Israf, Maha Abdullah |
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| Rok vydání: | 2010 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide T cell Immunology Cell Count Cell Communication Nitric Oxide Cell Line Mice chemistry.chemical_compound Bone Marrow medicine Animals Immunology and Allergy CD40 Antigens Cell Proliferation Pharmacology Mice Inbred ICR CD40 Microglia biology Mesenchymal stem cell Mesenchymal Stem Cells Coculture Techniques Cell biology medicine.anatomical_structure chemistry biology.protein Neuroglia Bone marrow Stem cell |
| Zdroj: | International Immunopharmacology. 10:1532-1540 |
| ISSN: | 1567-5769 |
| Popis: | The immunoregulatory properties of mesenchymal stem cells (MSC) have been demonstrated on a wide range of cells. Here, we describe the modulatory effects of mouse bone marrow-derived MSC on BV2 microglia proliferation rate, nitric oxide (NO) production and CD40 expression. Mouse bone marrow MSC were co-cultured with BV2 cells at various seeding density ratios and activated with lipopolysaccharide (LPS). We show that MSC exert an anti-proliferative effect on microglia and are potent producers of NO when stimulated by soluble factors released by LPS-activated BV2. MSC suppressed proliferation of both untreated and LPS-treated microglia in a dose-dependent manner, significantly reducing BV2 proliferation at seeding density ratios of 1:0.2 and 1:0.1 (p |
| Databáze: | OpenAIRE |
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