Type 1 Diabetes Is Associated With Enterovirus Infection in Gut Mucosa
| Autor: | Sisko Tauriainen, Sami Oikarinen, Katri Kaukinen, Immo Rantala, Maarit Oikarinen, Heikki Hyöty, Pekka Collin, Markku Mäki, Teemu Honkanen |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Adolescent viruses Endocrinology Diabetes and Metabolism 030209 endocrinology & metabolism Inflammation In situ hybridization Biology medicine.disease_cause Polymerase Chain Reaction Pathophysiology Virus 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Enterovirus Infections Internal Medicine medicine Humans Intestinal Mucosa In Situ Hybridization Tropism Aged Enterovirus 030304 developmental biology 0303 health sciences Type 1 diabetes Pancreatic islets HLA-DR Antigens medicine.disease 3. Good health Diabetes Mellitus Type 1 medicine.anatomical_structure Immunology RNA Viral Female medicine.symptom |
| Zdroj: | Diabetes; Vol 61 Diabetes |
| ISSN: | 1939-327X 0012-1797 |
| Popis: | Enterovirus infections have been linked to type 1 diabetes in several studies. Enteroviruses also have tropism to pancreatic islets and can cause β-cell damage in experimental models. Viral persistence has been suspected to be an important pathogenetic factor. This study evaluates whether gut mucosa is a reservoir for enterovirus persistence in type 1 diabetic patients. Small-bowel mucosal biopsy samples from 39 type 1 diabetic patients, 41 control subjects, and 40 celiac disease patients were analyzed for the presence of enterovirus using in situ hybridization (ISH), RT-PCR, and immunohistochemistry. The presence of virus was compared with inflammatory markers such as infiltrating T cells, HLA-DR expression, and transglutaminase 2–targeted IgA deposits. Enterovirus RNA was found in diabetic patients more frequently than in control subjects and was associated with a clear inflammation response in the gut mucosa. Viral RNA was often detected in the absence of viral protein, suggesting defective replication of the virus. Patients remained virus positive in follow-up samples taken after 12 months’ observation. The results suggest that a large proportion of type 1 diabetic patients have prolonged/persistent enterovirus infection associated with an inflammation process in gut mucosa. This finding opens new opportunities for studying the viral etiology of type 1 diabetes. |
| Databáze: | OpenAIRE |
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