Assessment for Microsatellite DNA Instability in Nasal Cytology Samples of Patients with Allergic Rhinitis
| Autor: | Emmanuel S. Helidonis, Maria I. Zervou, Katerina D. Samara, George A. Velegrakis, Alexander D. Karatzanis, Nikolaos M. Siafakas, Eleni G. Tzortzaki |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Genetic Markers Male medicine.medical_specialty Rhinitis Allergic Perennial Cytodiagnosis Loss of Heterozygosity Polymerase Chain Reaction Gastroenterology Loss of heterozygosity Atopy 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Humans 030223 otorhinolaryngology Asthma business.industry Rhinitis Allergic Seasonal Microsatellite instability Middle Aged medicine.disease Nasal Mucosa Otorhinolaryngology Genetic marker Nasal cytology 030220 oncology & carcinogenesis Sputum Microsatellite Female Microsatellite Instability medicine.symptom business |
| Zdroj: | American Journal of Rhinology. 21:236-240 |
| ISSN: | 1539-6290 1050-6586 |
| DOI: | 10.2500/ajr.2007.21.2956 |
| Popis: | Background Genetic alterations, including microsatellite instability (MSI) and loss of heterozygosity (LOH), have been described in both malignant and benign diseases. Previous studies have successfully detected such alterations in sputum samples of patients with bronchial asthma (BA). The aim of this study was to assess the presence of MSI and/or LOH in nasal cytology samples of patients with allergic rhinitis (AR). Methods Nasal brush samples and peripheral blood from 20 patients with AR were analyzed. DNA was extracted and analyzed for MSI and LOH using the following microsatellite markers: D16S289, D4S2394, D4S1651, DXS8039, D3S3606, and D2S2113, harboring potential susceptibility genes for AR and atopy. Microsatellite analysis was performed also in eight control subjects. Results No MSI and/or LOH were noted in either the AR or the control group. Conclusion Although MSI and LOH are detectable phenomena in sputum samples of patients with BA, this seems not to be the case for nasal cytology samples of patients with AR. Additional studies are needed, using a larger number of polymorphic markers, to assess if such a difference exists among two diseases otherwise very closely related. |
| Databáze: | OpenAIRE |
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