Determination of asparagine and glutamine in polypeptides using bis(I,I-trifluoroacetoxy)iodobenzene
| Autor: | Lynn M. Soby, Peter Johnson |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Stereochemistry
medicine.drug_class Glutamine Lysine Iodobenzene Biophysics Carboxamide Guanidines Biochemistry chemistry.chemical_compound Ribonucleases Aspartic acid medicine Insulin Asparagine Amino Acids Molecular Biology Iodobenzenes Hydrolysis Cell Biology Glutamic acid Actins Chymotrypsinogen chemistry Acid hydrolysis Peptides |
| Zdroj: | Analytical Biochemistry. 113:149-153 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(81)90058-0 |
| Popis: | A simple yet accurate method is described by which the numbers of asparagine and glutamine residues in polypeptides can be determined. The method involves difference analysis of the aspartic acid and glutamic acid contents of the polypeptide after acid hydrolysis (in 6 n HCl), without and with prior treatment of the sample with bis(I,I-trifluoroacetoxy)iodobenzene. Under the conditions described, this reagent quantitatively converts the carboxamide residues to the corresponding amines, which are eluted near (and interfere with the estimation of) the lysine peak on conventional ion-exchange amino acid analysis. During the carboxamide conversion, certain amino acid residues sensitive to oxidation are partially or completely destroyed and cannot be accurately determined. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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