Cellular Viral Rebound after Cessation of Potent Antiretroviral Therapy Predicted by Levels of Multiply Spliced HIV-1 RNA Encoding nef
| Autor: | Marek Fischer, Beda Joos, Gabriela Bleiber, Huldrych F. Günthard, Rainer Weber, Bernard Hirschel |
|---|---|
| Přispěvatelé: | University of Zurich, Fischer, Marek, Swiss HIV Cohort Study |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Neutrophils/virology
Neutrophils Anti-HIV Agents/ therapeutic use viruses Rev Gene Products Human Immunodeficiency Virus HIV Infections Gene Products tat/metabolism Virus Replication Gene Products nef Recurrence Immunology and Allergy Cells Cultured ddc:616 biology virus diseases Drug holiday Infectious Diseases Gene Products rev Gene Products rev/metabolism Lentivirus Gene Products tat 2723 Immunology and Allergy RNA Viral tat Gene Products Human Immunodeficiency Virus Drug Therapy Combination HIV-1/genetics/ isolation & purification HIV Infections/blood/ drug therapy/virology Anti-HIV Agents RNA Splicing Viremia 610 Medicine & health Gene Products nef/analysis/ biosynthesis RNA Viral/analysis/ biosynthesis Peripheral blood mononuclear cell 142-005 142-005 Virus In vivo medicine Humans nef Gene Products Human Immunodeficiency Virus Tat Gene Products Human Immunodeficiency Virus RNA rev Gene Products Human Immunodeficiency Virus 2725 Infectious Diseases biology.organism_classification medicine.disease Virology Leukocytes Mononuclear/ virology Viral replication Immunology HIV-1 Leukocytes Mononuclear Anti-HIV Agents/therapeutic use Gene Products nef/analysis Gene Products nef/biosynthesis HIV Infections/blood HIV Infections/drug therapy HIV Infections/virology HIV-1/genetics HIV-1/isolation & purification Leukocytes Mononuclear/virology RNA Viral/analysis RNA Viral/biosynthesis 570 Life sciences Nef Gene Products Human Immunodeficiency Virus |
| Zdroj: | The Journal of Infectious Diseases, Vol. 190, No 11 (2004) pp. 1979-1988 Journal of Infectious Diseases, vol. 190, no. 11, pp. 1979-1988 |
| ISSN: | 0022-1899 |
| Popis: | To characterize newly arising replication of human immunodeficiency virus (HIV) type 1 in vivo at the cellular level, distinct viral RNA species in peripheral blood mononuclear cells (PBMCs) from HIV-1-infected patients were monitored during 2 weeks of structured treatment interruption (STI). HIV-1 RNA encoding tat/rev and PBMC-associated virions were almost completely depleted during antiretroviral therapy and emerged simultaneously after 2 weeks of STI, thus specifically reflecting productive viral infection at the cellular level. The magnitude of these correlates of reappearing cellular viral replication was predicted by during-therapy levels of nef transcripts in PBMCs. Significant rebound of plasma viremia, representing the progeny of a broader range of anatomical compartments, preceded and predicted productive infection in PBMCs. Thus, cellular viral rebound in PBMCs likely was primed before STI by the expression of nef in HIV-1-infected PBMCs that lacked virion production and was subsequently triggered by the plasma viremia that preceded the recurrence of productively infected PBMCs. |
| Databáze: | OpenAIRE |
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