Pro-apoptotic and immunostimulatory tetrahydroxanthone dimers from the endophytic fungus Phomopsis longicolla
| Autor: | Christoph Janiak, Alexandra Hamacher, Richard Sawadogo, Stefanie Scheu, Peter Proksch, Amal H. Aly, Sebastian Wesselborg, Tibor Kurtán, Björn Stork, David Rönsberg, Philip Böhler, Attila Mándi, Matthias U. Kassack, Wenhan Lin, Abdessamad Debbab, Victor Wray, Vera Vasylyeva, Laura H. Engelke, Marc Diederich |
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| Rok vydání: | 2013 |
| Předmět: |
Models
Molecular Circular dichroism Stereochemistry T-Lymphocytes Xanthones Antineoplastic Agents Apoptosis Mice Structure-Activity Relationship Immune system Ascomycota Cell Line Tumor Animals Humans Phomopsis longicolla Cell Proliferation biology Dose-Response Relationship Drug Molecular Structure Activator (genetics) Chemistry Macrophages Organic Chemistry biology.organism_classification Semisynthesis Killer Cells Natural Cell culture Cancer cell Leukocytes Mononuclear Quantum Theory Dimerization |
| Zdroj: | The Journal of organic chemistry. 78(24) |
| ISSN: | 1520-6904 |
| Popis: | Four tetrahydroxanthone dimers (1-4) and four biogenetically related monomers (5-8), including the new derivatives 4-6, were isolated from the endophyte Phomopsis longicolla. The absolute configurations of 2-4 were established for the first time by TDDFT electronic circular dichroism calculations, and that of phomoxanthone A (1) was revised by X-ray crystallography. Phomoxanthone A (1) showed the strongest pro-apoptotic activity when tested against a panel of human cancer cell lines, including cisplatin-resistant cells, whereas it was up to 100-fold less active against healthy blood cells. It was also the most potent activator of murine T lymphocytes, NK cells, and macrophages, suggesting an activation of the immune system in parallel to its pro-apoptotic activity. This dual effect in combating cancer cells could help in fighting resistance during chemotherapy. Preliminary structure-activity studies of isolated compounds and derivatives obtained by semisynthesis (9a-11) hinted at the location of the biaryl axis and the presence of acetyl groups as important structural elements for the biological activity of the studied tetrahydroxanthones. |
| Databáze: | OpenAIRE |
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