Coffee constituents as modulators of Nrf2 nuclear translocation and ARE (EpRE)-dependent gene expression
| Autor: | Thomas Hofmann, Veronika Somoza, Nicole Teller, Ute Boettler, Doris Marko, Katharina Sommerfeld, Gudrun Pahlke, Roman Lang, Nadine Volz |
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| Rok vydání: | 2011 |
| Předmět: |
Antioxidant
Transcription Genetic NF-E2-Related Factor 2 Endocrinology Diabetes and Metabolism medicine.medical_treatment Blotting Western Clinical Biochemistry Quinic Acid Gene Expression Antineoplastic Agents Pyridinium Compounds Chromosomal translocation Oxidative phosphorylation Response Elements Coffee environment and public health digestive system Biochemistry Antioxidants chemistry.chemical_compound Caffeic Acids Western blot Trigonelline Gene expression medicine Humans Molecular Biology Cell Nucleus Nutrition and Dietetics medicine.diagnostic_test Plant Extracts Activator (genetics) respiratory system Oxidative Stress Protein Transport chemistry Pyridinium Chlorogenic Acid HT29 Cells Signal Transduction |
| Zdroj: | The Journal of Nutritional Biochemistry. 22:426-440 |
| ISSN: | 0955-2863 |
| DOI: | 10.1016/j.jnutbio.2010.03.011 |
| Popis: | Oxidative cellular stress initiates Nrf2 translocation into the nucleus, thus inducing antioxidant response element (ARE)-mediated expression of Phase II enzymes involved in detoxification and antioxidant defence. We investigated whether coffee extracts (CEs) of different proveniences and selected constituents have an impact on the Nrf2/ARE pathway in human colon carcinoma cells (HT29). Assessed as increased nuclear Nrf2 protein, Nrf2 nuclear translocation was modulated by different CEs as observed by Western blot analysis. In addition to the known Nrf2 activator 5-O-caffeoylquinic acid (CGA), pyridinium derivatives like the N-methylpyridinium ion (NMP) were identified as potent activators of Nrf2 nuclear translocation and ARE-dependent gene expression of selected antioxidative Phase II enzymes in HT29. Thereby, the substitution pattern at the pyridinium core structure determined the impact on Nrf2-signalling. In contrast, trigonelline was found to interfere with Nrf2 activation, effectively suppressing the NMP-mediated induction of Nrf2/ARE-dependent gene expression. In conclusion, several coffee constituents, partly already present in the raw material as well as those generated during the roasting process, contribute to the Nrf2-translocating properties of consumer-relevant coffee. A fine tuning in the degradation/formation of activating and deactivating constituents of the Nrf2/ARE pathway during the roasting process appears to be critical for the chemopreventive properties of the final coffee product. |
| Databáze: | OpenAIRE |
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