A multifunctional silk coating on additively manufactured porous titanium to prevent implant-associated infection and stimulate bone regeneration

Autor: B. van der Wal, A H Mirzaei, S. Amin Yavari, F. Jahanmard, Z Gorgin Karaji
Rok vydání: 2020
Předmět:
Electrophoresis
Staphylococcus aureus
Bone Regeneration
Prosthesis-Related Infections
Cell Survival
Surface Properties
0206 medical engineering
Silk
Biomedical Engineering
Fibroin
Bioengineering
02 engineering and technology
engineering.material
Surface engineering
Prosthesis Design
Bone and Bones
Biomaterials
Mice
Anti-Infective Agents
Coated Materials
Biocompatible
Coating
Osteogenesis
Vancomycin
Spectroscopy
Fourier Transform Infrared
Electrochemistry
Animals
MC3T3
Viability assay
Bone regeneration
Cell Proliferation
Titanium
Osteoblasts
Chemistry
Cell Differentiation
Implant Infection
3T3 Cells
Prostheses and Implants
Bombyx
021001 nanoscience & nanotechnology
020601 biomedical engineering
Anti-Bacterial Agents
Oxygen
engineering
Implant
0210 nano-technology
Porosity
Biomedical engineering
Zdroj: Biomedical Materials. 15:065016
ISSN: 1748-605X
1748-6041
Popis: Despite tremendous progress in the design and manufacturing of metallic implants, they do not outlive the patient. To illustrate, more than half of hip replacements will fail, mainly due to implant infection and loosening. Surface engineering approaches and, in particular, coatings can facilitate implant bio-functionality via the recruitment of more host cells for new bone formation and inhibition of bacterial colonization. Here, we used electrophoretic deposition to apply a silk fibroin solution consisting of tricalcium phosphate (TCP) and vancomycin as a coating on the surface of additively-manufactured porous titanium. Furthermore, the surface properties of the coatings developed and the release kinetics of the vancomycin were studied to evaluate the applied coating. The in vitro antibacterial behavior of the multifunctional coating, as well as the cell viability and osteogenic differentiation of the MC3T3-E1 cell line were extensively studied. The biomaterials developed exhibited an antibacterial behavior with a reduction of up to four orders of magnitude in both planktonic and adherent bacteria for 6 h and 1 d. A live-dead assay, the Alamar Blue activity, the DNA content, and cytoskeleton staining demonstrated a significant increase in the cell density of the coated groups versus the as-manufactured ones. The significantly enhanced calcium deposition and the increase in mineralization for the groups with TCP after 21 and 28 d, respectively, demonstrate upregulation of the MC3T3 cells’ osteogenic differentiation. Our results collectively show that the multifunctional coating studied here can be potentially used to develop a new generation of orthopedic implants.
Databáze: OpenAIRE
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